CNA04830 (C. neoformans )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CNA04830
Gene Name:  Ptc1p
Original Clone ID:
9021a1BCt5p1
Keyword:
None
Species:
Cryptococcus neoformans
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
FnCD00312202
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1176nts
Open reading frame: 51 to 1226

TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTTCTAATTTAC
CGGCGGATTCGTTTCGTGTGGGCGTTAGTGAGGACCGCAACCGTCGCTGCCGTCGTACCA
TGGAGGATGCCCATTCATTCGTCTATGACTTTGCAGGTGTAAAAGGGCAGGGATACTTCG
CTGTGTTTGATGGCCACGCGGGTAAACATGCCGCAGAATGGTGTGGGCAAAATTTCCACG
AGTATCTGCTGGACGGACTGCTGATTGAACCAGATACGCCTGTTCCGGATCTGATGAACA
AAACTTTTCATCTGGTCGACAAACGCCTGTCGCACCTGGCTCATGCGGCCGGCACATCGA
GCGGTTGCACCGCAGTAACGGCTTTCCTGCGCGTGGAAGAGATGGATGACAATGATAGCT
TCCGTAAAGGCTTCACTAACCCAGGTCTGCAGCCTCGCGGGCTGTGGGAAGGAAAAGGCG
AGGACGAACTGGAAAGTCAAACATCACTGCAGCCGTCCTCGCGTCGCAGCTCTATGGGTG
GCGGTACCGGGGGACAGATGGGCGGTGCGAGTGCCCCGGGGGTTAATGGCCGTACGGGTT
CACTGGCACGCCGTCCGAGTTCGAAAAAAATCCGCGATTTTGTCAAAGGACTGACCGGCG
GTATGGAGAAAAACTTTGATGAAGCTATTACTGAGGACGAAGGGGTGATCAGCGCGGCCG
ATGGCACACGTGTAGAAGCAATTGAGCCAAAATCTGAAAAAGGTGTTCGCCGTGTGCTGT
ACACGGCGAATGTCGGGGACGCTCGCGCCGTTCTGTGTCGTGGCGGTAAAGCAGTACGCC
TGACCTATGATCACAAAGGGAGTGATGCGCAAGAGGCTAAACGTATCACTGACGCCGGAG
GCTTCGTGATGAACAATCGCGTCAACGGTGTTCTGGCAGTGACACGTTCACTGGGGGATG
CGTCCATGAAAGAATTTGTAGTCGGCGCTCCTTACACCACGGAAACCACTCTGGACGAGC
AGGATGAGTTCCTGATTGTTGCCTGCGACGGTCTGTGGGATGTGTGTAGCGATCAAGAAG
CAGTAGAGATCGTTCATCTGGTGACAGACCCGCAGGAAGCGTCGAAACGCCTGCTGGATC
ACGCCATGTCTAATTTTAGTACGGACAACCTGTCAGTCATGGTTGTGCGTTTCAATCATG
AAGGGCATCATCACCATCACCACTGATCGGAATTCGAGCTCCGTCGACAAGCTTGCGGCC
GCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

SNLPADSFRVGVSEDRNRRCRRTMEDAHSFVYDFAGVKGQGYFAVFDGHAGKHAAEWCGQ
NFHEYLLDGLLIEPDTPVPDLMNKTFHLVDKRLSHLAHAAGTSSGCTAVTAFLRVEEMDD
NDSFRKGFTNPGLQPRGLWEGKGEDELESQTSLQPSSRRSSMGGGTGGQMGGASAPGVNG
RTGSLARRPSSKKIRDFVKGLTGGMEKNFDEAITEDEGVISAADGTRVEAIEPKSEKGVR
RVLYTANVGDARAVLCRGGKAVRLTYDHKGSDAQEAKRITDAGGFVMNNRVNGVLAVTRS
LGDASMKEFVVGAPYTTETTLDEQDEFLIVACDGLWDVCSDQEAVEIVHLVTDPQEASKR
LLDHAMSNFSTDNLSVMVVRFNHEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t566c,L189P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9021a
Recommended expression in:
Not Applicable