DNASU Plasmid | WISP1 (Homo sapiens) in pSGX4

Explanation of Terms

Gene:

Gene Symbol: WISP1

Gene Name: WNT1 inducible signaling pathway protein 1

Original Clone ID:

13035a6BSt3p1

Keyword:

full-length cds 26-367 of 367 aa

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSGX4

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

"N-terminal 6-His and Smt3, cleavable, TOPO"

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00312292

Price:

AVAILABLE

No restriction
Special MTA: PSI/Smt3/SUMO

Insert Sequence
Protein Sequence

Insert sequence: 1035nts

Open reading frame: 51 to 1085

Reference Sequence Alignment

AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTTCTCCAGCCC
CTACGACCATGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCT
GTAAATGGCCTTGTGAGTGCCCACCAAGCCCACCCCGTTGCCCCCTGGGTGTATCGCTCA
TCACTGACGGCTGTGAATGCTGTAAGATGTGCGCTCAGCAGCTTGGGGATAATTGTACAG
AAGCCGCGATTTGTGACCCACATCGTGGCCTCTACTGTGATTATAGCGGGGACCGCCCGA
GGTACGCAATAGGAGTGTGTGCACAGGTGGTCGGCGTCGGTTGTGTTCTGGATGGTGTTC
GTTACAACAACGGCCAGTCGTTTCAACCCAATTGTAAGTATAACTGCACCTGTATCGACG
GCACCGTGGGTTGCACCCCACTGTGCTTGCGCGTACGTCCCCCGCGTCTCTGGTGTCCTC
ATCCGCGGCGCGTATCTATCCCGGGTCATTGCTGTGAACAGTGGGTATGTGAGGACGACG
CCAAGAGGCCCCGTAAGACCGCACCCCGTGACACAGGAGCCTTCGATGCTGTGGGTGAGG
TGGAGGCATGGCACAGGAACTGCATAGCCTACACAAGCCCCTGGAGCCCTTGCTCCACCA
GCTGCGGCCTGGGTGTCTCAACTCGGATCTCCAATGTTAACGCCCAGTGCTGGCCTGAGC
AAGAGAGCCGCCTCTGCAACTTGCGTCCGTGCGATGTGGACATCCATACACTCATTAAGG
CAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATGAACTTCACTTTGGCTG
GCTGTATCTCAACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGT
GCTGTATTCCTTACAAGTCTAAGACTATCGACGTTAGCTTCCAGTGCCCTGATGGGCTTG
GCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATC
CCAATGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACGAAG
GGTAACTCGAGCACCACCACCACCACCACTGAGATCCGGCTCTAACAAGCCCGAAG

Protein sequence predicted based on nucleotide sequence

SPAPTTMDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLGVSLITDGCECCKMCAQQLGD
NCTEAAICDPHRGLYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYNNGQSFQPNCKYNCT
CIDGTVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDA
VGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHT
LIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCP
DGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIANEG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g373a,A125T

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in NYSGXRC-13035a

Recommended expression in:

Not Applicable

Vector Name:

pSGX4

Synonyms:

pSGX-4, pSGX4_BS

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.

Size (bp):

5611

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTAAGGGTAACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

TOPO recognition site

TOPO

TOPO cloning site

5435

5444

bacterial origin

ColE

ColE1 bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4984

5003

protease cleavage site

ULP site

ULP cleavage site (recognizes the tertiary structure of SUMO)

5140

repressor protein gene

LacI

lacI cds

3515

4351

ribosome binding site

RBS

ribosome binding site (RBS)

5062

5068

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

Smt3

Smt3 tag

5140

5424

tag

His

6xHis tag

5083

5100

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 termination sequence

5539

5585

Gene

Gene Symbol :

WISP1

Symbol Nomenclature :

WISP1

SYNONYM :

CCN4; WISP1c; WISP1i; WISP1tc

Designation :

CCN family member 4|CTC-458A3.8|WNT1 induced secreted protein 1|WNT1-inducible-signaling pathway protein 1|Wnt-1 inducible signaling pathway protein 1

Full Nomenclature :

WNT1 inducible signaling pathway protein 1

GENEID :

8840

GI :

4507921

GenBank Accession :

NM_003882

HGNC :

12769

MIM :

603398

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00312292      -------------------------AGGATAACGATATTATTGAGGCTCACAGAGAACAG
NM_003882.3       ATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTC
                                           :**.:...*: * :. :*..**  .***.... : 

HsCD00312292      ATTGGTGGATCCCTTTCTCCAGCCCCTACGACCATGGACTTTACCCCAGCTCCACTGGAG
NM_003882.3       CTGGCCACGGCCCTCTCTCCAGCCCCTACGACCATGGACTTTACCCCAGCTCCACTGGAG
                  .* *  . . **** *********************************************

HsCD00312292      GACACCTCCTCACGCCCCCAATTCTGTAAATGGCCTTGTGAGTGCCCACCAAGCCCACCC
NM_003882.3       GACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCC
                  ************************** **.*****:***********.***: *******

HsCD00312292      CGTTGCCCCCTGGGTGTATCGCTCATCACTGACGGCTGTGAATGCTGTAAGATGTGCGCT
NM_003882.3       CGCTGCCCGCTGGGGGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCT
                  ** ***** ***** **.:  ********:** ********.******************

HsCD00312292      CAGCAGCTTGGGGATAATTGTACAGAAGCCGCGATTTGTGACCCACATCGTGGCCTCTAC
NM_003882.3       CAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGCCTCTAC
                  ************** ** ** **.**.** ** ** ********.** ** *********

HsCD00312292      TGTGATTATAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACAGGTGGTCGGC
NM_003882.3       TGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACAGGTGGTCGGT
                  ***** ** ************************************************** 

HsCD00312292      GTCGGTTGTGTTCTGGATGGTGTTCGTTACAACAACGGCCAGTCGTTTCAACCCAATTGT
NM_003882.3       GTGGGCTGCGTCCTGGATGGGGTGCGCTACAACAACGGCCAGTCCTTCCAGCCTAACTGC
                  ** ** ** ** ******** ** ** ***************** ** **.** ** ** 

HsCD00312292      AAGTATAACTGCACCTGTATCGACGGCACCGTGGGTTGCACCCCACTGTGCTTGCGCGTA
NM_003882.3       AAGTACAACTGCACGTGCATCGACGGCGCGGTGGGCTGCACACCACTGTGCCTCCGAGTG
                  ***** ******** ** *********.* ***** *****.********* * **.**.

HsCD00312292      CGTCCCCCGCGTCTCTGGTGTCCTCATCCGCGGCGCGTATCTATCCCGGGTCATTGCTGT
NM_003882.3       CGCCCCCCGCGTCTCTGGTGCCCCCACCCGCGGCGCGTGAGCATACCTGGCCACTGCTGT
                  ** ***************** ** ** ***********.:  **.** ** ** ******

HsCD00312292      GAACAGTGGGTATGTGAGGACGACGCCAAGAGGCCCCGTAAGACCGCACCCCGTGACACA
NM_003882.3       GAGCAGTGGGTATGTGAGGACGACGCCAAGAGGCCACGCAAGACCGCACCCCGTGACACA
                  **.********************************.** *********************

HsCD00312292      GGAGCCTTCGATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCTACACA
NM_003882.3       GGAGCCTTCGATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCTACACA
                  ************************************************************

HsCD00312292      AGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCCTGGGTGTCTCAACTCGGATCTCCAAT
NM_003882.3       AGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAAT
                  ************************************** *****.***************

HsCD00312292      GTTAACGCCCAGTGCTGGCCTGAGCAAGAGAGCCGCCTCTGCAACTTGCGTCCGTGCGAT
NM_003882.3       GTTAACGCCCAGTGCTGGCCTGAGCAAGAGAGCCGCCTCTGCAACTTGCGGCCATGCGAT
                  ************************************************** **.******

HsCD00312292      GTGGACATCCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAG
NM_003882.3       GTGGACATCCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAG
                  ************************************************************

HsCD00312292      GCATCCATGAACTTCACTTTGGCTGGCTGTATCTCAACACGCTCCTATCAACCCAAGTAC
NM_003882.3       GCATCCATGAACTTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTAC
                  *****************: * ** ***** ***: .************************

HsCD00312292      TGTGGAGTTTGCATGGACAATAGGTGCTGTATTCCTTACAAGTCTAAGACTATCGACGTT
NM_003882.3       TGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGACTATCGACGTG
                  ***************************** ** ** *********************** 

HsCD00312292      AGCTTCCAGTGCCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGC
NM_003882.3       TCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGC
                  : ********* ************************************************

HsCD00312292      TTCTGTAACCTGAGCTGTAGGAATCCCAATGACATCTTTGCTGACTTGGAATCCTACCCT
NM_003882.3       TTCTGTAACCTGAGCTGTAGGAATCCCAATGACATCTTTGCTGACTTGGAATCCTACCCT
                  ************************************************************

HsCD00312292      GACTTCTCAGAAATTGCCAACGAAGGGTAACTCGAGCACCACCACCACCACCACTGAGAT
NM_003882.3       GACTTCTCAGAAATTGCCAACTAG------------------------------------
                  ********************* *.                                    

HsCD00312292      CCGGCTCTAACAAGCCCGAAG
NM_003882.3       ---------------------