PRUNE (Homo sapiens) in pSGX4

Explanation of Terms

Gene:
Gene Symbol:  PRUNE
Gene Name:  prune exopolyphosphatase
Original Clone ID:
13028a12BSt20p1
Keyword:
partial length cds 14-401 of 453 aa
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSGX4
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"N-terminal 6-His and Smt3, cleavable, TOPO"
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HsCD00312304
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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 1173nts
Open reading frame: 51 to 1223

AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTGAGTCCCGAC
CTCTACATGTTGTGCTGGGAAATGAAGCCTGTGATTTGGACTCCACAGTGTCTGCTCTTG
CCCTGGCTTTTTACCTAGCAAAGACAACTGAGGCTGAGGAAGTCTTTGTGCCAGTTTTAA
ATATAAAACGTTCTGAACTACCTCTGCGAGGTGACATTGTCTTCTTTCTTCAGAAGGTTC
ATATTCCAGAGAGTATCTTGATTTTTCGGGATGAGATTGACCTCCATGCATTATACCAGG
CTGGCCAACTCACCCTCATCCTTGTCGACCATCATATCTTATCCAAAAGTGACACAGCCC
TAGAGGAGGCAGTAGCAGAGGTGCTAGACCATCGACCCATCGAGCCGAAACACTGCCCTC
CCTGCCATGTTTCAGTTGAGCTGGTGGGGTCCTGTGCTACCCTGGTGACCGAGAGAATCC
TGCAGGGGGCACCAGAGATCTTGGACAGGCAAACTGCAGCCCTTCTGCATGTAACCATCA
TCCTGGACTGTGTCAACATGGACCTTAAAATTGGAAAGGCAACCCCAAAGGACAGCAAAT
ATGTGGAGAAACTAGAGGCCCTTTTCCCAGACCTACCCAAGAGAAATGATATATTTGATT
CCCTACAAAAGGCAAAGTTTGATGTATCAGGACTGACCACTGAGCAGATGCTGAGAAAAG
ACCAGAAGACTATCTATAGACAAGGCGTCAAGGTGGCCATTAGTGCAATATATATGGATT
TGGAGGCCTTTCTGCAGAGGTCTAACCTCCTTGCAGATCTCCATGCTTTCTGCCAGGCTC
ACAGCTATGATGTCCTGGTTGCCATGACTATCTTTTTCAACACTCACAATGAGCCAGTGC
GGCAGTTGGCTATTTTCTGTCCCCATGTGGCACTCCAAACAACGATCTGTGAAGTCCTGG
AACGCTCCCACTCTCCACCCCTGAAGCTGACCCCTGCCTCAAGTACCCACCCTAACCTCC
ATGCCTATCTTCAAGGCAACACCCAGGTCTCTCGAAAGAAACTTCTGCCCCTGCTCCAGG
AAGCCCTGTCAGCATATTTTGACTCCATGAAGATCCCTTCAGGACAGCCTGAGACAGCAG
ATGTGTCCAGGGAGCAAGTGGACAAGGAATTGGACAGGGCAAGTAACTCCCTGATTTCTG
GACTGAGTCAAGATGAAGGGTAACTCGAGCACCACCACCACCACCACTGAGATCCGGCTG
TAACAAGCCCGAAG

Protein sequence predicted based on nucleotide sequence

ESRPLHVVLGNEACDLDSTVSALALAFYLAKTTEAEEVFVPVLNIKRSELPLRGDIVFFL
QKVHIPESILIFRDEIDLHALYQAGQLTLILVDHHILSKSDTALEEAVAEVLDHRPIEPK
HCPPCHVSVELVGSCATLVTERILQGAPEILDRQTAALLHVTIILDCVNMDLKIGKATPK
DSKYVEKLEALFPDLPKRNDIFDSLQKAKFDVSGLTTEQMLRKDQKTIYRQGVKVAISAI
YMDLEAFLQRSNLLADLHAFCQAHSYDVLVAMTIFFNTHNEPVRQLAIFCPHVALQTTIC
EVLERSHSPPLKLTPASSTHPNLHAYLQGNTQVSRKKLLPLLQEALSAYFDSMKIPSGQP
ETADVSREQVDKELDRASNSLISGLSQDEG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g482t,G161V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-13028a
Recommended expression in:
Not Applicable