SETD8 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SETD8
Gene Name:  SET domain containing (lysine methyltransferase) 8
Original Clone ID:
1193.1.53_GO.37140
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00312787
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1107nts
Open reading frame: 1 to 1107

ATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCTAGAGGC
AGGAAGATGTCCAAGCCCCGCGCGGTGGAGGCGGCGGCGGCGGCGGCGGCGGTGGCAGCG
ACGGCCCCGGGCCCGGAGATGGTGGAGCGGAGGGGCCCGGGGAGGCCCCGCACCGACGGG
GAGAACGTATTTACCGGGCAGTCAAAGATCTATTCCTACATGAGCCCGAACAAATGCTCT
GGAATGCGTTTCCCCCTTCAGGAAGAGAACTCAGTTACACATCACGAAGTCAAATGCCAG
GGGAAACCATTAGCCGGAATCTACAGGAAACGAGAAGAGAAAAGAAATGCTGGGAACGCA
GTACGGAGCGCCATGAAGTCCGAGGAACAGAAGATCAAAGACGCCAGGAAAGGTCCCCTG
GTACCTTTTCCAAACCAAAAATCTGAAGCAGCAGAACCTCCAAAAACTCCACCCTCATCT
TGTGATTCCACCAATGCAGCCATCGCCAAGCAAGCCCTGAAAAAGCCCATCAAGGGCAAA
CAGGCCCCCCGAAAAAAAGCTCAAGGAAAAACGCAACAGAATCGCAAACTTACGGATTTC
TACCCTGTCCGAAGGAGCTCCAGGAAGAGCAAAGCCGAGCTGCAGTCTGAAGAAAGGAAA
AGAATAGATGAATTGATTGAAAGTGGGAAGGAAGAAGGAATGAAGATTGACCTCATCGAT
GGCAAAGGCAGGGGTGTGATTGCCACCAAGCAGTTCTCCCGGGGTGACTTTGTGGTGGAA
TACCACGGGGACCTCATCGAGATCACCGACGCCAAGAAACGGGAGGCTCTGTACGCACAG
GACCCTTCCACGGGCTGCTACATGTACTATTTTCAGTATCTGAGCAAAACCTACTGCGTG
GATGCAACTAGAGAGACAAATCGCCTAGGAAGACTGATCAATCACAGCAAATGTGGGAAC
TGCCAAACCAAACTGCACGACATCGACGGCGTACGTCACCTCATCCTCATCGCCTCCCGA
GACATCGCGGCTGGGGAGGAGCTCCTGTATGACTATGGGGACCGCAGCAAGGCTTCCATT
GAAGCCCACCCGTGGCTGAAGCATTAA

Protein sequence predicted based on nucleotide sequence

MPTLYKKAGSENLYFQSARGRKMSKPRAVEAAAAAAAVAATAPGPEMVERRGPGRPRTDG
ENVFTGQSKIYSYMSPNKCSGMRFPLQEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNA
VRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGK
QAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLID
GKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCV
DATRETNRLGRLINHSKCGNCQTKLHDIDGVRHLILIASRDIAAGEELLYDYGDRSKASI
EAHPWLKH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.37140
Recommended expression in:
Not Applicable