Dennd1b (Mus musculus) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Dennd1b
Gene Name:  DENN/MADD domain containing 1B
Original Clone ID:
1189.1.62_GO.35898
Keyword:
full-length cds
Species:
Mus musculus
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
MmCD00313035
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 717nts
Open reading frame: 51 to 767

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGACCGCAGGACCAGGGAAAATCCAGAAAGAACTTTTGACTTGGTATTGA
AAGTGAAGTGTCATGCCTCTGAAAATGAAGATCCTGAGGTATTGTGGAAATTCCCAGAGG
ACTTTGGAGACCAGGAAGTTCTGCAGAGCGTGCCCAAGTTCTGTTTTCCCTTCGATGTGG
AAAGGGTGTCTCAGAATCAAGTTGGACAGCACTTTACCTTCGTCCTGACAGACATGGAAA
GTAAGCAGCGATTTGGGTTCTGCAGGCTCACATCTGGAGGCAGAATTTGTTTATGTATTC
TTAGTTACTTGCCATGGTTTGAAGTATATTATAAACTTCTAAATACTCTGGCAGATTACT
TGGCCAAGGAACTGGAAGAGGATTTGAATGAAACTCTTAAATCACTCTATAACCACCCAG
TACCAAAGGCAAACACTCCTGTCAACTTGAGTGTGAACCAAAAGCTATTTATTGCCAGTG
AGCAAGTTCTGAAAGATGACCCCTCACTAATTCCGCACTCCTGTTTCATCACACCTGACA
TAACTGGACTCCCAACGATACCGGAGAGTAGAAACCTGACTGAGTACTTTGTTGCCGTGG
ATGTCAACAACATGCTGCGACTGTACGCCAGCATGCTGCATGAGAGGCGCATCATTATTA
CCTCGAGCAAATTAAGCACTGTAAGTCCTTCACACTTGCGCCTTTGATAGGACCCAGCTT
TCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSDRRTRENPERTFDLVLKVKCHASENEDPEVLWKFPEDFGDQEVLQSVPKFCFP
FDVERVSQNQVGQHFTFVLTDMESKQRFGFCRLTSGGRICLCILSYLPWFEVYYKLLNTL
ADYLAKELEEDLNETLKSLYNHPVPKANTPVNLSVNQKLFIASEQVLKDDPSLIPHSCFI
TPDITGLPTIPESRNLTEYFVAVDVNNMLRLYASMLHERRIIITSSKLSTVSPSHLRL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g472a,E158K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.35898
Recommended expression in:
Not Applicable