Nsmce2 (Mus musculus) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Nsmce2
Gene Name:  non-SMC element 2 homolog (MMS21, S. cerevisiae)
Original Clone ID:
1189.1.93_GO.35966
Keyword:
full-length cds
Species:
Mus musculus
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
MmCD00313083
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 762nts
Open reading frame: 51 to 812

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCCCAGGACGGTCCAGTACAAGTTCAGGTTCTACCCGTTACATATCCTTCA
GTGGCATAGAGTCAGCTCTCTCCTCCTTAAAAAACTTCCAATCCTGTATCAGCTCTGGAA
TGGACACAGTTTCTAGTGTTGCCTTGGACCTTGTGGAGACTCAAACTGAAGTGAGTAGTG
AGTACAGTATGGACAAGGCCATGGTTGAGTTTGCTAAAATGGATCGAGAACTAAGCCATT
ATGTTAAGGCTGTGCAGTCTACAATCAATCATGTAAAAGAAGAACGTCCAGAAAAAGTAC
CAGATTTAAAATTACTGGTAGAGAAGAAATTTTTGGCTTTACAGGATAAGAACTCTGATG
CCGACTTTAAAGAGAATGAAAAGTTCGTGCAGTTCAAGCAACAGCTGAGGGAGCTGAAGA
AGCAATATGGTATTCATGCAGACAGAGAGAATGATCTGACAGAAGGAGTTGACGAAGATA
TGATTGTGACCCAGAGCCAAACCAATTTCATCTGCCCCATAACACAGCTGGAAATGAAGA
AGCCAGTGAAAAATAAAATGTGTGGCCATACATACGAAGAGGAAGCCATTGTTCGCATGA
TTGAATCCAAGCATAAGAGGAAGAAGAAGGCCTGTTGCCCCAAAATTGGCTGTAGCCACA
CAGACATGAGAATGTCAGATCTCATTCCGGATGAAGCCCTGAGAAGGGCAATCGAGAGCC
ACAACAAGAAGAAAAAACGCCACTCCGAATAGTAGGACCCAGCTTTCTTGTACAAAGTTG
GCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSPGRSSTSSGSTRYISFSGIESALSSLKNFQSCISSGMDTVSSVALDLVETQTE
VSSEYSMDKAMVEFAKMDRELSHYVKAVQSTINHVKEERPEKVPDLKLLVEKKFLALQDK
NSDADFKENEKFVQFKQQLRELKKQYGIHADRENDLTEGVDEDMIVTQSQTNFICPITQL
EMKKPVKNKMCGHTYEEEAIVRMIESKHKRKKKACCPKIGCSHTDMRMSDLIPDEALRRA
IESHNKKKKRHSE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.35966
Recommended expression in:
Not Applicable