ANNAT1 (A. thaliana Columbia )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ANNAT1
Gene Name:  annexin 1
Original Clone ID:
5541.1.76_GO.4020
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00313431
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 972nts
Open reading frame: 51 to 1022

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCGACTCTTAAGGTTTCTGATTCTGTTCCTGCTCCTTCTGATGATGCTG
AGCAATTGAGAACCGCTTTTGAAGGATGGGGTACGAACGAGGACTTGATCATATCAATCT
TGGCTCACAGAAGTGCTGAACAGAGGAAAGTCATCAGGCAAGCATACCACGAAACCTACG
GCGAAGACCTTCTCAAGACTCTTGACAAGGAGCTCTCTAACGATTTCGAGAGAGCTATCT
TGTTGTGGACTCTTGAACCCGGTGAGCGTGATGCTTTATTGGCTAATGAAGCTACAAAAA
GATGGACTTCAAGCAACCAAGTTCTTATGGAAGTTGCTTGCACAAGGACATCAACGCAGC
TGCTTCACGCTAGGCAAGCTTACCATGCTCGCTACAAGAAGTCTCTTGAAGAGGACGTTG
CTCACCACACTACCGGTGACTTCAGAAAGCTTTTGGTTTCTCTTGTTACCTCATACAGGT
ACGAAGGAGATGAAGTGAACATGACATTGGCTAAGCAAGAAGCTAAGCTGGTCCATGAGA
AAATCAAGGACAAGCACTACAATGATGAGGATGTTATTAGAATCTTGTCCACAAGAAGCA
AAGCTCAGATCAATGCTACTTTTAACCGTTACCAAGATGATCATGGCGAGGAAATTCTCA
AGAGTCTTGAGGAAGGAGATGATGATGACAAGTTCCTTGCACTTTTGAGGTCAACCATTC
AGTGCTTGACAAGACCAGAGCTTTACTTTGTCGATGTTCTTCGTTCAGCAATCAACAAAA
CTGGAACTGATGAAGGAGCACTCACTAGAATTGTGACCACAAGAGCTGAGATTGACTTGA
AGGTCATTGGAGAGGAGTACCAGCGCAGGAACAGCATTCCTTTGGAGAAAGCTATTACCA
AAGACACTCGTGGAGATTACGAGAAGATGCTCGTCGCACTTCTCGGTGAAGATGATGCTT
AATAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSATLKVSDSVPAPSDDAEQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYH
ETYGEDLLKTLDKELSNDFERAILLWTLEPGERDALLANEATKRWTSSNQVLMEVACTRT
STQLLHARQAYHARYKKSLEEDVAHHTTGDFRKLLVSLVTSYRYEGDEVNMTLAKQEAKL
VHEKIKDKHYNDEDVIRILSTRSKAQINATFNRYQDDHGEEILKSLEEGDDDDKFLALLR
STIQCLTRPELYFVDVLRSAINKTGTDEGALTRIVTTRAEIDLKVIGEEYQRRNSIPLEK
AITKDTRGDYEKMLVALLGEDDA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.4020
Recommended expression in:
Not Applicable