CASP8 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DACH2
Gene Name:  dachshund homolog 2 (Drosophila)
Original Clone ID:
5545.1.55_GO.56036
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00313702
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 855nts
Open reading frame: 51 to 905

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAG
ATCTGGCCTCCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCA
TCAAGGATGCCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCA
ATCTGTCCTTCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCT
ACCTAAACACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCAAA
TTTCTGCCTACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATTGA
GGTCTTTTAAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGA
ACCTGCTGGATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGAGAAGGAAAGTTGG
ACATCCTGAAAAGAGTCTGTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACT
ATGAAGAATTCAGCAAAGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGACTCTCCAA
GAGAACAGGATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTC
GGGGATACTGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCA
AACTTCACAGCATTAGGGACAGGAATGGAACACACTTGGATGCAGGGTCTCACTCTGTTG
CCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCACTGCAGCCTCCGCCTCCCTGGTTCG
GGTGATAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRML
EESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSR
SELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKI
INDYEEFSKGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKARE
KVPKLHSIRDRNGTHLDAGSHSVAQAGVQWCDLGSLQPPPPWFG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.56036
Recommended expression in:
Not Applicable