Gene:
Gene Symbol:
AT4G14520
Gene Name:
DNA-directed RNA polymerase II-like protein
Original Clone ID:
5476.1.08_GO.16684
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
AtCD00313776
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 603nts
Open reading frame: 51 to 653
ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCTCGAGACGTGGCTATATGTGCCAAACACTTGAATGGCCAATCGCCGC
ACCAACCGATTCTATGCAGACTACTCCAAGATCTAATACACGAGAAAGCGTGCAGGGAAC
ACGGATTCTATCTCGGCATCACCGCTCTAAAAAGCATCGGTAACAACAAGAACAACAACA
TCGACAACGAAAACAATCATCAAGCAAAAATCCTCACATTCCCGGTGTCCTTCACATGCC
GCACATTCTTGCCTGCAAGAGGAGATATCTTGCAAGGGACTGTAAAAAAAGTGTTGTGGA
ACGGAGCTTTTATCAGGTCAGGACCACTCAGATACGCATACCTCTCACTCTTGAAAATGC
CACATTATCATTATGTCCACTCACCATTGTCGGAGGACGAGAAACCGCATTTTCAGAAAG
ACGACCTCTCAAAGATTGCTGTTGGAGTTGTTGTGCGGTTTCAGGTGTTGGCTGTGCGGT
TTAAAGAGAGACCACACAAGAGAAGAAACGACTATTATGTTCTTGCAACTTTGGAAGGCA
ATGGTTCATTTGGTCCCATCTCTCTTACAGGGTCAGATGAGCCATACATGTAATAGGACC
CAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA
Protein sequence predicted based on nucleotide sequence
ENLYFQSARDVAICAKHLNGQSPHQPILCRLLQDLIHEKACREHGFYLGITALKSIGNNK NNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYLSL LKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVLAT LEGNGSFGPISLTGSDEPYM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.16684
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
