Gene:
Gene Symbol:
CD630_10690
Gene Name:
histidinol phosphatase/hydrolase
Original Clone ID:
APC61519_SG139_pMCSG9
Keyword:
full length
Species:
Clostridioides difficile 630
Type:
cDNA
Vector Name:
pMCSG9
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
CdCD00321723
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 795nts
Open reading frame: 13 to 807
CAATCCAATGCCATGTTTTACGACTATCACATGCACTCAAGTTTCTCTACAGACGGAAAA
TCTACAATGGAAGAAATGGTAAAAAAATCTATTGAACTCGGTTTGGAGGAAATTTGTTTC
ACAGACCATGTTGACTATGATGTATATGCTGATGATTCTTTTTCAATTGTCTACGAAGAT
TATTTTAAGAGTTTAGAGACTCTTCAAAGCAAATATAAAGATAAAATATCAATAAAAAAA
GGAATTGAATTTGGAGTTCAAACTCAATTAATGGATACATACAAAAAGGAAGCACATCAA
TATCCATTGGACTTTATAATATGTTCTATCCATGCTATAGATACAATGGACTTATACCTT
GGTAATTATTTTAAAGATAAAACTCAACATGAAGTTTATGAAAATTACTATCTTTATTTA
TATAATATTGTTAAGAATTATAAAGATTACTCTGTCTTAGGTCATTTAGATTTGATTAAA
AGGTATGCACCTTATGACACTATTTTAGATGATAGACTATTTTCTGATATAATTGAAGAA
ACTCTTAAACAAGCTATTTATGATGGCAAAGGTATCGAGATAAATACCTCTTGTTATAGA
TATAATCTTCCAGACCTAACCCCATCTAAGTATATACTTCAAATGTACAAAGACTTGGGA
GGGGAAATCATAACCACAGGTTCAGATTCACATCATATAAGTCAAGTTGCATGTGAGTTT
GACTACATATATTCATTACTAAAAAATATGGGATTTAAATATGTATCTAAATTTAATAAA
TTGAAACCAGAATTTATAAAATTATAACATTGGAAGTGGATAACGGATCCGAATTCGAGC
TCCGTCGACAAGCTTGCG
Protein sequence predicted based on nucleotide sequence
MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDVYADDSFSIVYEDYFKS LETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFIICSIHAIDTMDLYLGNYF KDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAPYDTILDDRLFSDIIEETLKQ AIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGEIITTGSDSHHISQVACEFDYIY SLLKNMGFKYVSKFNKLKPEFIKL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Tested_Not_Found
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC61519
Recommended expression in:
Not Applicable
Vector Name:
pMCSG9
Synonyms:
pMCSG-9
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative
Size (bp):
6405
Parent Vector:
None
Properties:
bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID:
16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) site
204
235
bacterial origin
ori
pBR322-type bacterial origin of replication
4399
4399
promoter
T7
T7 promoter
1479
1495
protease cleavage site
TEV
TEV protease cleavage site cds
223
243
repressor binding site
lac operator
lacO regulatory sequence
1454
1476
repressor protein gene
LacI
LacI cds
2009
2968
selectable marker
AmpR
ampicillin resistance
5157
6017
tag
His
6xHis tag
140
157
tag
His 2
N-terminal 6xHis tag
1387
1404
tag
MBP
maltose binding protein cds
265
1362
trxn termination sequence
T7 term
T7 terminator
26
72
