meis2.1 (Danio rerio) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  meis2b
Gene Name:  myeloid ecotropic viral integration site 2b
Original Clone ID:
1257.1.91_GO.72116
Keyword:
None
Species:
Danio rerio
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
DrCD00321763
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1200nts
Open reading frame: 51 to 1250

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCCTGATGGCTCAACGGTACGAAGACCTTGCCCACTATGGTGGCGGGATGG
ATGGAGTTGGGGTTCCGACGTCGATGTACGGGGACCCGCACGCGCCTCGGCCCCTGCCGC
AGGTTCATCATTTGAATCACGGGCCGCCACCACATGCCAACCAACACTACGGGGCCCACG
CACCACACAGCATCATGCCCAGCAGCATGGGCTCAGCTGTCAACGACGCACTTAAAAGAG
ACAAGGACCAAATTTACGGGCACCCGTTATTCCCGCTGCTGGCGCTGGTGTTTGAGAAGT
GCGAGCTGGCCACATGCACACCGAGAGAGCCGGGAGTCGCTGGAGGAGATGTGTGTTCAT
CTGATTCATTTAACGAGGACATCGCTGTGTTTGCCAAACAGATTCGTGCAGAGAAGCCGT
TATTTTCCTCTAACCCAGAGCTGGATAATTTGATGATTCAGTCAATACAAGTATTACGAT
TTCATCTTTTAGAACTCGAAAAGGTGCACGAGCTTTGCGACAATTTCTGCCATCGGTATA
TTAGCTGTTTGAAGGGCAAAATGCCCATTGACCTAGTAATTGATGAACGGGATGGATGCA
AATCTGACTTCGACGACCTCTCAGGATCATCAACAAATCTCGCAGATCACAATCCAGCAT
CCTGGAGAGACATGGACGACGCTCACTCCACTCCCTCAGTTGGCACCCCGGGACCCTCCA
GCGGAGGACATGCCTCCCAGAGCGGAGACAACAGCAGTGAACTAGGAGATGGCTTGGATA
ACAGCTTGGCATCTCCGGGAACTGGGGATGAGGACGATCAGGACAAAAAACGGCAGAAGA
AAAGAGGAATCTTCCCCAAAGTGGCGACCAACATCATGCGGGCATGGCTCTTCCAACATC
TCACGCACCCCTACCCATCTGAAGAGCAGAAGAAGCAGCTAGCACAAGACACAGGCCTCA
CCATCTTACAAGTGAATAACTGGTTTATCAATGCTCGAAGAAGAATCGTGCAGCCCATGA
TTGATCAGTCTAACAGAGCCGTAAGTCAAGGAACCGCTTACAGCCCTGATGGTCAGCCTA
TGGGCGGCTTTGTGCTAGATGGCCAACAACACATGGGTCTTCGGCCTGGCGGTCCCATGG
GAGGAATGGGGATGAACATGGGTATGGATGGACAGTGGCACTACATGTAATAGGACCCAG
CTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSLMAQRYEDLAHYGGGMDGVGVPTSMYGDPHAPRPLPQVHHLNHGPPPHANQHY
GAHAPHSIMPSSMGSAVNDALKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGD
VCSSDSFNEDIAVFAKQIRAEKPLFSSNPELDNLMIQSIQVLRFHLLELEKVHELCDNFC
HRYISCLKGKMPIDLVIDERDGCKSDFDDLSGSSTNLADHNPASWRDMDDAHSTPSVGTP
GPSSGGHASQSGDNSSELGDGLDNSLASPGTGDEDDQDKKRQKKRGIFPKVATNIMRAWL
FQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVSQGTAYSPD
GQPMGGFVLDGQQHMGLRPGGPMGGMGMNMGMDGQWHYM*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.72116
Recommended expression in:
Not Applicable