CV_3890 (C. violaceum ATCC 12472 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CV_3890
Gene Name:  spore coat polysaccharide biosynthesis protein E
Original Clone ID:
11105i1BCt8p1
Keyword:
partial length cds 2-340 of 495 aa
Species:
Chromobacterium violaceum ATCC 12472
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
CvCD00321860
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1044nts
Open reading frame: 51 to 1094

ATCCTCAGAATATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTAGCAAGCCGC
TATTCATCTTCGAGATGGCCAACAACCACATGGGCAACGTCGAGCACGGCGTCGCGCTGA
TCCGCGCCATCCGCGAATCCTGCCAGGGCTTCGATTTCGACTTCGGCTTCAAGCTGCAGT
ACCGCAACCTGGACACCTTCATCCATTCCAGCTTCAAGGGCCGCGACGACGTCAAGTACG
TGAAGCGTTTCGAGGAGACCCGGCTGCAGCCGGAACAGATGCAAAAGCTGGTGGCCGAGA
TGAAGGCCAACGGCTTCAAGGCGATCTGCACCCCGTTCGACGAGGAATCGGTGGACCTGA
TCGAAGCGCACGGCATCGAGATCATCAAGATCGCCAGTTGCTCGTTCACCGATTGGCCCC
TGCTGGAGCGCATCGCCCGCAGCGACAAGCCGGTGGTGGCGTCCACCGCCGGCGCGCGGC
GCGAGGACATCGACAAGGTGGTCAGCTTCATGCTGCATCGCGGCAAGGATCTGACCATCA
TGCACTGTGTGGCCGAGTACCCGACGCCGGACGACCATCTGCACCTGGCGCGGATCAAGA
CGTTGCGCCAGCAGTACGCCGGCGTGCGCATCGGCTACTCCACCCACGAAGACCCGGACT
TGATGGAACCCATCATGCTGGCGGTCGCCCAGGGCGCGACGGTGTTCGAGAAGCATGTGG
GCCTGCCCACCGACCAGTACGGCATCAACAATTACTCCGCCAATCCGGAGCAGGTCCGCC
GCTGGCTGGCCGCCGCCGCGCGCGCGCTGGCCATGCTGGGCGATGGCGAGGACGACGCGG
TGTCGGAGACCGAGCAAGCCAGCCTGCGCTCGCTGCGCCGCGGCGTGTTCGCCACCCGGC
CGGTCGCGGCCGGCGAGGCGCTGACCGCCGACAATGTCAGCTTCGCCTTCCCGCCGGTGG
AAGGGCAACTGACCGCCAACGAATGGTCCAAATATGTCCGCTACACCGCCAAGACGCCGA
TCGCCGCCGACGCGCCCGTGATGGCCGCCGATCTGGAGCCGGTGAACGAAGGGCATCATC
ACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCAC
CACCA

Protein sequence predicted based on nucleotide sequence

SKPLFIFEMANNHMGNVEHGVALIRAIRESCQGFDFDFGFKLQYRNLDTFIHSSFKGRDD
VKYVKRFEETRLQPEQMQKLVAEMKANGFKAICTPFDEESVDLIEAHGIEIIKIASCSFT
DWPLLERIARSDKPVVASTAGARREDIDKVVSFMLHRGKDLTIMHCVAEYPTPDDHLHLA
RIKTLRQQYAGVRIGYSTHEDPDLMEPIMLAVAQGATVFEKHVGLPTDQYGINNYSANPE
QVRRWLAAAARALAMLGDGEDDAVSETEQASLRSLRRGVFATRPVAAGEALTADNVSFAF
PPVEGQLTANEWSKYVRYTAKTPIAADAPVMAADLEPVNEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11105i
Recommended expression in:
Not Applicable