kdsD (R. pomeroyi DSS-3 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  kdsD
Gene Name:  arabinose 5-phosphate isomerase
Original Clone ID:
45459
Keyword:
None
Species:
Ruegeria pomeroyi DSS-3
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SpCD00323973
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 969nts
Open reading frame: 51 to 1019

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGACCGATC
GCAGCTCTTTCATCGACACCGCACGCCAGGTCGTGCTGGACGAGGCCCGGGCGCTTGATG
CGCTGTCCGAGAGCCTGGGCGACGGGTTCGCCGAGGCGGTGGAGCTGATCCTGCGCACCA
AGGGGCGCGTGATCGTTTCGGGCATTGGCAAGTCGGGCCATATCGGCCACAAGATCGCCG
CGACGCTGGCCAGCACCGGCACGCCCGCCTATTTCGTGCACCCGGCCGAGGCGAGCCATG
GCGATCTGGGAATGCTCTCCGGGGATGACGTGGTTCTGGCCATCTCGAACTCGGGCGAGG
CGCCGGAACTGGCCAATCTGCTGGCCTTTACCCGCCGCTTCGCCATTCCGCTGATCGGGC
TGTCGAGCAAATCTGAGAGCTCGCTGATGCAGCAGGCCGATGTGCATCTGCTGATCCCGG
CGCTGGGCGAGGCCTGCGGCTTTGGCATGGTGCCGTCGATCTCGACCACGCTGACGCTGG
CCATGGGCGACGCACTGGCCATCGCGCTGATGAAGTACCGCGATTTCCGGCCCGAGAACT
TCCGCGTCTTCCATCCCGGCGGCAAGCTGGGCGCGCGGCTCAGCCGGGTGTCAGACCTGA
TGCATGGCGGCGAGGCGGTACCGTTGATCGCCGCCGACACCCCGATGAGCGAGGCGCTGC
TGGAGATTAGCCGCAAGGGGTTTGGCGTGGTCGGCGTGACCGATGGCGCCGGACATCTGG
CCGGAATCGTCACCGATGGCGACCTGCGTCGTCACATGTCGGGCCTGCTCGACAAGACCG
CCGCCCAGGTGATGACCGCCAACCCGACCACCATCGCCCCCGACTCGCTGGCCGAGGAAG
CGGTGGCTATCATGAACGCGCGCAAGATCACCTCGCTTTTCGTGGTCGATCCGGCGGAAC
CGGGCGTGGCGCGCGGGCTGCTGCATATCCACGACTGTCTGCGCGTCGGACTGGGCTAAC
GCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MTDRSSFIDTARQVVLDEARALDALSESLGDGFAEAVELILRTKGRVIVSGIGKSGHIGH
KIAATLASTGTPAYFVHPAEASHGDLGMLSGDDVVLAISNSGEAPELANLLAFTRRFAIP
LIGLSSKSESSLMQQADVHLLIPALGEACGFGMVPSISTTLTLAMGDALAIALMKYRDFR
PENFRVFHPGGKLGARLSRVSDLMHGGEAVPLIAADTPMSEALLEISRKGFGVVGVTDGA
GHLAGIVTDGDLRRHMSGLLDKTAAQVMTANPTTIAPDSLAEEAVAIMNARKITSLFVVD
PAEPGVARGLLHIHDCLRVGLG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 391450
Recommended expression in:
Not Applicable