Mpe_A2762 (M. petroleiphilum PM1 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Mpe_A2762
Gene Name:  hypothetical protein
Original Clone ID:
47025
Keyword:
None
Species:
Methylibium petroleiphilum PM1
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 23852666
Title: Crystal structure of a member of a novel family of dioxygenases (PF10014) reveals a conserved cupin fold and active site
PMID: 23852666
Title: Crystal structure of a member of a novel family of dioxygenases (PF10014) reveals a conserved cupin fold and active site
Authors:
PSI
Joint Center for Structural Genomics
MpCD00325753
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 762nts
Open reading frame: 51 to 812

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCACGTCG
ATATCGAACTGCCCCTCGGCCGCGCCACCGCGCTGCAGCGTCTGCGCGCCCAGGGTTTCT
GCGTGTTGACGCCGGCCGCGCTGGAAACGCTGACCGGCATGCCGCTCGACGCCTTCGACA
TGATGCTGCCGTACTGGGAAGAGCTGGCGCCGGACCTGCACCTCAAGGATGGCGGCCACT
ACCGCTACCGCCGTCACGGCTGCTTCATGCAGACCCTGCAGCCGGGGCAGCTCGAGACCG
TGCAGCACCGCGCCCACTGGCAACCCACCACCTACAACGCGCTGCATGGCGGCATGGAGC
GCTGGTTCGAGCCGCTGTCGAACGAGATGATCCACCTGCCTTCGTGGAGCGCCCTGCTGG
TTGCGCTCGGCGAGCTGTTCGCCAAGCTGCGCGCGCCGCAGGGCGGCCGCTGGTACATCG
AGGCCCATCCCTTCCGCATCGACACCGAAGGGGGCGTGGGGCGGCCGACGCCCGAAGGCG
CTCACCGCGATGGCGTCGACTTCGTCGCGGTGGTGTTCATCGGCCGGCAGGGCGTGCGCG
GCGGCGAGACACGGGTATTCGACGCGGCGGGCCCGCAGGGCGTGCGCTTCACGCTCGAGC
AGCCCTGGACCGTGCTGCTGCTCGACGATCAGCAGGTGATCCACGAAAGCACGCCGCTGT
TGCCGCTGGACCCCGCCGACCCGGCGGTGCCGGCCCATCGCGACACGCTGGTCCTGACCT
ACCGCAGCGGAGGCTTCCAGGCGCCGGCCTAACGCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MHVDIELPLGRATALQRLRAQGFCVLTPAALETLTGMPLDAFDMMLPYWEELAPDLHLKD
GGHYRYRRHGCFMQTLQPGQLETVQHRAHWQPTTYNALHGGMERWFEPLSNEMIHLPSWS
ALLVALGELFAKLRAPQGGRWYIEAHPFRIDTEGGVGRPTPEGAHRDGVDFVAVVFIGRQ
GVRGGETRVFDAAGPQGVRFTLEQPWTVLLLDDQQVIHESTPLLPLDPADPAVPAHRDTL
VLTYRSGGFQAPA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 392100
Recommended expression in:
Not Applicable