Sfri_2489 (S. frigidimarina NCIMB 400 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Sfri_2489
Gene Name:  metal dependent phosphohydrolase
Original Clone ID:
48318
Keyword:
None
Species:
Shewanella frigidimarina NCIMB 400
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SfCD00326290
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1251nts
Open reading frame: 51 to 1301

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAAAAAA
ACCAAAAAATCTCTGTAGAACAGTTAGAAGTGGGCACCTTTATCCGCTTGCCAATTTCCT
GGAAAGACCACCCTTTCCTGTTTAGCAGTTTTAAAATTAAAGACGCTGCACAAATAGAAC
TCATCAAAGGCTTAGGCCTAAAAGATGTTTTTTTTGATCCCGAAAAAAGTGATGTTGAAC
CTGTAACGTCCGAGAATGCCAGCACCCAAAAACCCGACGAGCAGGCCCTCAACGCGCTAA
AAGAACAAATGATTAGCCAGAAGTCGGCTAAAATCGATGAGCAACAAAAGCTCAAACGAG
ATTTAAAGAAAACTGAACAACAATTTAATCGTTCTGTGTCGATGATGCGTTCAATGGTGT
CTAAAATCAGTAGCCGACCACTCAATGCGGTCAGTGAAGCGAAAGACCTCATCAGCAACA
TGACCACCATGTTACTCAATAGTGATAATTTAGCCTTACATTTAATGGGCGATGCTAAAA
ATGATGAGCATATTTATCACCATTCACTTAACGTCGCTATGTTATGTATGTTAATGGCAA
AAGAGCTAGGTTGGAGCCGCGAAGAAGTTGAAACGATTGGTATTGGTGGGTTATTTCACG
ATATAGGTAAACTCAAAGTTCCAAGCAACATTCTCAACAAGCGAGTGCCGCTTACCACGC
CAGAAGAAAACTTTATTAAACAGCACCCATTGATGAGCATTAACTTTCTTAAACTCGCTG
ACAGCTTTCCAGATGAAGCCAAACCGTTAATTGCTAATCATCACGAATATCTAGATGGCA
GTGGCTACCCACAAGGGCTCAAAGCCGACAAATTAGACAAGCTGTCGCAGTTGATTGCCG
TGGTTAACGAATACGACAATTTGTGTAATGGCACCAACCAAGCTAAAGGGAAACCACCCA
GTATCGCCTTGGGGTTGCTGTATAAAAACTATAAAAACAAACTCAATGTTGAATATATTA
GCAAGTTAATCAAAATGTTAGGTATTTATCCGCCTGGCAGTATTATTGAATTATCCAGTG
GCCAATTTGCTATGGTTATGTCGGTAAACTTACAGAAGCTATTGTGCCCTAATATTGTGG
TTTACGATCAACTGGTACCCAAAGATTTAGCCCCCATCATTAGTTTACAAGATGAAGGCT
TGAGCATTGTACGCAGTCTACCCGCCGCAGCATTGCCAGAAAAAATCCATAAATACCTCA
ATCCTAGAGAACAAATCAGCTTAGGAATTGGTCAAAACTAACGCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MKKNQKISVEQLEVGTFIRLPISWKDHPFLFSSFKIKDAAQIELIKGLGLKDVFFDPEKS
DVEPVTSENASTQKPDEQALNALKEQMISQKSAKIDEQQKLKRDLKKTEQQFNRSVSMMR
SMVSKISSRPLNAVSEAKDLISNMTTMLLNSDNLALHLMGDAKNDEHIYHHSLNVAMLCM
LMAKELGWSREEVETIGIGGLFHDIGKLKVPSNILNKRVPLTTPEENFIKQHPLMSINFL
KLADSFPDEAKPLIANHHEYLDGSGYPQGLKADKLDKLSQLIAVVNEYDNLCNGTNQAKG
KPPSIALGLLYKNYKNKLNVEYISKLIKMLGIYPPGSIIELSSGQFAMVMSVNLQKLLCP
NIVVYDQLVPKDLAPIISLQDEGLSIVRSLPAAALPEKIHKYLNPREQISLGIGQN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 394041
Recommended expression in:
Not Applicable