SO_0281 (S. oneidensis MR-1 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  pilM
Gene Name:  type IV pilus biogenesis protein PilM
Original Clone ID:
48470
Keyword:
None
Species:
Shewanella oneidensis MR-1
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SoCD00326357
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1080nts
Open reading frame: 51 to 1130

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCTTTCAA
ATTTATGGAAGCGTCAGGCTCCGCAAATGGTCGGGATTGATATTGGTTCCCATGAAGTAA
AGGCCATTTTGCTGGGTAAGACAGCTGATGGATACAAAATTTTAAGTCATGCAGCGGTTC
CCGTAAAAAAGGGGGCAATTAATGATCATGATATTCGTGATGCTGATGCCGTTGTTGAAT
GTTTAAGACAGATTAAGCGCATTTTGCCTAAATCGGTAAAATATGCGGCTGTTGCGGTCT
CTGGCTCAGCGGTGATGACCAAGGTCATCTATATGGATGCTTCGCTTAGCGAAGAGGAAA
TGGAAGCGCAAATCGAGATTGAGGCCGATAACCTGATCCCTTATTCCCTTGATGAAGTCA
GCATTGATTTTGAAACGCTAAACGTCAACAGCACCGATCCATCTAAGGTCGATGTGCTAC
TCAGTGCTTGTCGTACCGACAATATCGATACCAGAGTCGATGCGCTCGATGAGGTTGAGC
TAGAAACCAAAGTCGTTGATGTTGAAGGTTATGCTTTAGGTCGAGCAGTTGAGTTAGTCC
TTGGGCAATTACCCGAAGGAGCTAGACAGAAAGCCGTCGCAATGGTTGATATCGGTGCGA
ATATGACGACTTTCTGCGTGGTAGAGTCCGGCGAAACCACCTTTATCCGTGAGCAAGCCT
TTGGTGGTGAGTTATTTACGCAGTCGATTCTCTCTTTTTACGGCATGTCCTACGAGCAAG
CCGAAAAAGCCAAAATCGAGGGGGATTTACCGCGTAATTATATGTTTGAAGTGTTATCTC
CCTTCCAAACGCAATTACTGCAACAAATTAAGCGAACACTGCAAATTTATTGCACCTCGA
GCGGCAAAGATAAAGTTGATTATCTCGTTTTATGCGGTGGAACCTCTAAACTGGAAGGTA
TGGCTAACTTATTGACCAATGAGTTAGGTGTCCACACCATTATTGCCGATCCTTTCCAAG
GGTGTCTGCATGCAGATGAGTCCGTTAAAAATATACTGCAACCTAGCATCAGCAAATATA
TGGTTGCTTGTGGCTTAGCGCTGAGGAGCTACGGTCAATGGCGAACATAACGCGACTTAA
TTAA

Protein sequence predicted based on nucleotide sequence

MLSNLWKRQAPQMVGIDIGSHEVKAILLGKTADGYKILSHAAVPVKKGAINDHDIRDADA
VVECLRQIKRILPKSVKYAAVAVSGSAVMTKVIYMDASLSEEEMEAQIEIEADNLIPYSL
DEVSIDFETLNVNSTDPSKVDVLLSACRTDNIDTRVDALDEVELETKVVDVEGYALGRAV
ELVLGQLPEGARQKAVAMVDIGANMTTFCVVESGETTFIREQAFGGELFTQSILSFYGMS
YEQAEKAKIEGDLPRNYMFEVLSPFQTQLLQQIKRTLQIYCTSSGKDKVDYLVLCGGTSK
LEGMANLLTNELGVHTIIADPFQGCLHADESVKNILQPSISKYMVACGLALRSYGQWRT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in 394145
Recommended expression in:
Not Applicable