Sama_0136 (S. amazonensis SB2B )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Sama_0136
Gene Name:  putative metal dependent phosphohydrolase
Original Clone ID:
48022
Keyword:
None
Species:
Shewanella amazonensis SB2B
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SaCD00326775
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1197nts
Open reading frame: 51 to 1247

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGCCGATA
GGGCATCTGAAGCCGAATTGATAAAGTTGCCTGTCAGCAAACTCACCCGAGGCATGTTTG
TGGCGGCTATTGAAAATAACGGCAAGGTCGCCGTTGCCAATGCCGGGCAAATCAAGAGCC
GGGAGACCATCGCCAAGCTGGTTAAAAGCGGTATTCATCATGTGTGGGTGGATGCAGAGC
GCTCCTCCGACGAATCCGGTCTCAAACCCAAGTCTGCCCAGGGCAGTGCAGCGCCAGCTG
CCGGTGCTGTTCGTCCTTCTCGCGCCAACCGCGACCTGCGTCAAACCCAGGCCAAGAAAC
TCCTCCATGAAGCCAAGGAACTTATCCGCAAGGTATTGTCCGAAACCTTCGAAGGCAAAG
CCATTGAAGTAAAGCCTTTTGAGGCGCTGGCCGACAATATGATTGAGTCAATCATGCTTG
ATGATGATGCCCTCAAATGTGTGTCGGCGCTGCGTGACAAAGATGCCTATCTGCTTGAAC
ATTCAGTGAATGTAGCCTTTCTGTTGGTCACCTTTGGCAAGTATTTGAAGCTCGATCGTG
AAACCTTAAAGCAGATGGCGGTGGGCGGTGTGCTGCACGACATAGGTAAAATCAAGGTTG
ATAACAAGATTTTGCATAAGCCCGGCAAGCTGACACCGGAAGAATTTGAGCACATGAAAC
TGCACCAGTTTTATGCGATGGACATTATGTCCGAAGCCTCGGCGCTGTCGCAGATTTCAA
AAGATGTATGCCTGATGCACCATGAAAAGCTCGATGGTCGCGGTTATCCCCGTGGTTTGA
AGGGCGATGAAATCCCACTCCACGGCCGCATGTCCTGTATCGTGGATATTTTCGATGCCC
TGACCGCCACCCGCTGCTACAAAGAGGCCATGAGCCCTGCGGCCGCCTTTAAGATCTTGC
TGAGTTTGACGCCTTTCCATCTGGACACCGATCTGGTGTACGAATTTATCCGCTGTATCG
GTGTCTATCCGGTGGGATCTCTGGTGGAGCTGTCCGACGGGCGTGTGGGCATTGTCTGGG
AGTCCAAAGACAGGGATGCCCTGAGCCCGGTAGTGAAATGTTTTTATTCACTCAAGCACA
AACGCTACACCGAAGTGAATTTTGTCGACCTGCTCAAGTCAGATCTGAACATAGAGCGGG
GCGTCTCCCCATCCAGCCTGGATGTAGACCCCAAGCCATTTTACTAACGCGACTTAATTA
A

Protein sequence predicted based on nucleotide sequence

MADRASEAELIKLPVSKLTRGMFVAAIENNGKVAVANAGQIKSRETIAKLVKSGIHHVWV
DAERSSDESGLKPKSAQGSAAPAAGAVRPSRANRDLRQTQAKKLLHEAKELIRKVLSETF
EGKAIEVKPFEALADNMIESIMLDDDALKCVSALRDKDAYLLEHSVNVAFLLVTFGKYLK
LDRETLKQMAVGGVLHDIGKIKVDNKILHKPGKLTPEEFEHMKLHQFYAMDIMSEASALS
QISKDVCLMHHEKLDGRGYPRGLKGDEIPLHGRMSCIVDIFDALTATRCYKEAMSPAAAF
KILLSLTPFHLDTDLVYEFIRCIGVYPVGSLVELSDGRVGIVWESKDRDALSPVVKCFYS
LKHKRYTEVNFVDLLKSDLNIERGVSPSSLDVDPKPFY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 394122
Recommended expression in:
Not Applicable