NIFL (W. succinogenes DSM 1740 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  NIFL
Gene Name:  nitrogen fixation positive protein
Original Clone ID:
APC38692.1_SG253_pMCSG19
Keyword:
partial cds
Species:
Wolinella succinogenes DSM 1740
Type:
cDNA
Vector Name:
pMCSG19
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
purifi_date: yield:
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Brian Feldmann
PSI
Midwest Center for Structural Genomics
WsCD00328370
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 738nts
Open reading frame: 51 to 788

TTTCTGGCGTGGATCTGGTTACCGAGAACCTGTACTTCCAATCCAATGCCCGCAATGGAC
AGATCAGGGAGGAGCTGGAGCGTTCCAAAATGATCACTCGAATGATTCAAGAGGCGATTC
GATCGGATTTTATTCAGGCTTATGGGCAGAAGATTGTGAGCGTTAAAGAGCCAAATCTCT
ACAAAATCGAAACCCTCATGAGGATTGTTGATGGGTCGGGCAAAATCACTTCGCCTTTTA
TCTTTTTGGAGCAGGCGAAAAACTCTAGACTCTATTCGCGCCTCACGCGCATCATGATCA
AAAAGGTTTTTGAGAGCTTTAAAGGGAATGCGCTGGAGTTCTCGATCAATCTCACCTTTG
AAGATATTTTAGATCATGAGACAACTCGATTTATTCTTGATTCTATTCAGTCGATGGGGC
TCCAAAATCGCGTCACCATTGAGCTAGTGGAGAGTGAAGAGATTGGAGCGAGTCCAGAGA
TGGAGCTGTTCATCAAAGAGGCCAAAGCATTGGGATGCAAAATCTCTATTGATGACTTTG
GAAGTGGTTACTCCAATTTTGATTACATCCTGAAAATCGGCGCCGATTTCATCAAGATTG
ATGGTTCGCTCATCAAAAACATTGACACCGACAAAAACTCCTATATCACGGTCAAGACGA
TCATCGCTTTGGCTAAAGAGCTTGGGATTGGCGTGGTGGCAGAGTATATTCACTCCAAAG
AGGTGATGGAGGTGGTGGTGGAGCTTGGGGTGGATTATCTGCAAGGGTATCATTTGCACG
AACCTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

RNGQIREELERSKMITRMIQEAIRSDFIQAYGQKIVSVKEPNLYKIETLMRIVDGSGKIT
SPFIFLEQAKNSRLYSRLTRIMIKKVFESFKGNALEFSINLTFEDILDHETTRFILDSIQ
SMGLQNRVTIELVESEEIGASPEMELFIKEAKALGCKISIDDFGSGYSNFDYILKIGADF
IKIDGSLIKNIDTDKNSYITVKTIIALAKELGIGVVAEYIHSKEVMEVVVELGVDYLQGY
HLHEP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: N/A
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC38692.1
Recommended expression in:
Not Applicable