Original Clone ID:
FLH269136.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.2/V5-DEST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00329401
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCT
GAAAACCAACAAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCT
ATGCCAGCAGCTCATGCAACATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAAT
GGGCAGACAGTTCAAGTCCATGGAGTCATTCAGGCGGCCCAGCCATCAGTTATTCAGTCT
CCACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAAAAAGACTTTTCTCCGGAACA
CAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGAT
TCCCAAAAGCGAAGGGAAATTCTTTCAAGGAGGCCTTCCTACAGGAAAATTTTGAATGAC
TTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACT
TCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTAGCAGTGGA
CAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGG
GTACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACC
ATTCTACAGTATGCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTT
GTTGTTCAAGCTGCCTCTGGAGACGTACAAACATACCAGATTCGCACAGCACCCACTAGC
ACTATTGCCCCTGGAGTTGTTATGGCATCCTCCCCAGCACTTCCTACACAGCCTGCTGAA
GAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGT
CGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAAT
CAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCA
GAT
Protein sequence predicted based on nucleotide sequence
MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPN GQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTD SQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSG QYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQV VVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAAREC RRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
1120
Start on reference sequence
95
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLenti6.2/V5-DEST
Synonyms:
pLenti6.2/V5-DEST
Sequencing Primer:
Forward:
CMV forward
Reverse:
V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with RSV and 5pLTR and HIV-1 psi and RRE and gp41 peptide and CMV enhancer and CMV Promoter and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and V5 and PGK and EM7 and BlastR and 3pLTR and SV40 and SV40 Ori and T7 promoter and M13 forward and f1 ori and AmpR promoter and AmpR and ori and CAP binding site and lac promoter and lac operator and M13 reverse and T3; blasticidin resistance in mammalian, ampicillin resistance in bacterial;
Comments:
None
Size (bp):
8812
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
3'UTR
5pLTR
truncated 5' long terminal repeat (LTR) from HIV-1
230
410
3pUTR
3pLTR
self-inactivating 3' long terminal repeat (LTR) from HIV-1
5333
5566
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
3674
3979
CDS
BlastR
confers resistance to blasticidin
4848
5246
CDS
CmR
confers resistance to chloramphenicol
2673
3332
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
6727
7587
CDS
V5
epitope tag from simian virus 5
4197
4238
CDS
gp41 peptide
antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)
1493
1537
Miscellaneous Feature
HIV-1 psi
packaging signal of human immunodeficiency virus type 1
457
582
Miscellaneous Feature
RRE
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
1075
1308
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
1816
2119
poly-A signal
SV40
SV40 polyadenylation signal
5638
5759
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
5983
5999
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
8732
8748
promoter
CMV Promoter
human cytomegalovirus (CMV) immediate early promoter
2120
2323
promoter
PGK
mouse phosphoglycerate kinase 1 promoter
4271
4768
promoter
RSV
Rous sarcoma virus enhancer/promoter
3
229
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2589
2619
promoter
EM7
synthetic bacterial promoter
4782
4829
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
5955
5973
promoter
AmpR promoter
null
6622
6726
promoter
lac promoter
promoter for the E. coli lac operon
8670
8700
promoter
T3
promoter for bacteriophage T3 RNA polymerase
8769
8787
protein binding site
attR1
recombination site for the Gateway LR reaction
2440
2564
protein binding site
attR2
recombination site for the Gateway LR reaction
4020
4144
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
8634
8655
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
8708
8724
rep_origin
SV40 Ori
SV40 origin of replication
5799
5934
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
6141
6596
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
7758
8346
Gene
Gene Symbol :
CREB1
Symbol Nomenclature :
CREB1
SYNONYM :
CREB
Designation :
CREB-1|active transcription factor CREB|cAMP-response element-binding protein-1|cAMP-responsive element-binding protein 1|cyclic AMP-responsive element-binding protein 1|transactivator protein
Full Nomenclature :
cAMP responsive element binding protein 1
GENEID :
HGNC :
MIM :
Vega :
Target GenBank:
Reference Sequence Alignment
HsCD00329401 ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCT NM_004379.3 ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCT NM_134442.3 ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCT ************************************************************ HsCD00329401 GAAAACCAACAAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCT NM_004379.3 GAAAACCAACAAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCT NM_134442.3 GAAAACCAACAAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCT ************************************************************ HsCD00329401 ATGCCAGCAGCTCATGCAACATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAAT NM_004379.3 ATGCCAGCAGCTCATGCAACATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAAT NM_134442.3 ATGCCAGCAGCTCATGCAACATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAAT ************************************************************ HsCD00329401 GGGCAGACAGTTCAAGTCCATGGAGTCATTCAGGCGGCCCAGCCATCAGTTATTCAGTCT NM_004379.3 GGGCAGACAGTTCAAGTCCATGGAGTCATTCAGGCGGCCCAGCCATCAGTTATTCAGTCT NM_134442.3 GGGCAGACAGTTCAAGTCCATGGAGTCATTCAGGCGGCCCAGCCATCAGTTATTCAGTCT ************************************************************ HsCD00329401 CCACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAAAAAGACTTTTCTCCGGAACA NM_004379.3 CCACAAGTCCAAACAGTTCAG--------------------------------------- NM_134442.3 CCACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAAAAAGACTTTTCTCCGGAACA ********************* HsCD00329401 CAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGAT NM_004379.3 ---ATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGAT NM_134442.3 CAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGAT ********************************************************* HsCD00329401 TCCCAAAAGCGAAGGGAAATTCTTTCAAGGAGGCCTTCCTACAGGAAAATTTTGAATGAC NM_004379.3 TCCCAAAAGCGAAGGGAAATTCTTTCAAGGAGGCCTTCCTACAGGAAAATTTTGAATGAC NM_134442.3 TCCCAAAAGCGAAGGGAAATTCTTTCAAGGAGGCCTTCCTACAGGAAAATTTTGAATGAC ************************************************************ HsCD00329401 TTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACT NM_004379.3 TTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACT NM_134442.3 TTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACT ************************************************************ HsCD00329401 TCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTAGCAGTGGA NM_004379.3 TCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTAGCAGTGGA NM_134442.3 TCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTAGCAGTGGA ************************************************************ HsCD00329401 CAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGG NM_004379.3 CAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGG NM_134442.3 CAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGG ************************************************************ HsCD00329401 GTACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACC NM_004379.3 GTACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACC NM_134442.3 GTACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACC ************************************************************ HsCD00329401 ATTCTACAGTATGCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTT NM_004379.3 ATTCTACAGTATGCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTT NM_134442.3 ATTCTACAGTATGCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTT ************************************************************ HsCD00329401 GTTGTTCAAGCTGCCTCTGGAGACGTACAAACATACCAGATTCGCACAGCACCCACTAGC NM_004379.3 GTTGTTCAAGCTGCCTCTGGAGACGTACAAACATACCAGATTCGCACAGCACCCACTAGC NM_134442.3 GTTGTTCAAGCTGCCTCTGGAGACGTACAAACATACCAGATTCGCACAGCACCCACTAGC ************************************************************ HsCD00329401 ACTATTGCCCCTGGAGTTGTTATGGCATCCTCCCCAGCACTTCCTACACAGCCTGCTGAA NM_004379.3 ACTATTGCCCCTGGAGTTGTTATGGCATCCTCCCCAGCACTTCCTACACAGCCTGCTGAA NM_134442.3 ACTATTGCCCCTGGAGTTGTTATGGCATCCTCCCCAGCACTTCCTACACAGCCTGCTGAA ************************************************************ HsCD00329401 GAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGT NM_004379.3 GAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGT NM_134442.3 GAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGT ************************************************************ HsCD00329401 CGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAAT NM_004379.3 CGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAAT NM_134442.3 CGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAAT ************************************************************ HsCD00329401 CAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCA NM_004379.3 CAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCA NM_134442.3 CAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCA ************************************************************ HsCD00329401 GATTTG NM_004379.3 GATTAA NM_134442.3 GATTAA ****:.
Pathways
NCI :
AP-1 transcription factor network
NCI :
ATF-2 transcription factor network
NCI :
ErbB1 downstream signaling
NCI :
FOXA2 and FOXA3 transcription factor networks
NCI :
Glucocorticoid receptor regulatory network
NCI :
HIF-1-alpha transcription factor network
NCI :
Integrin-linked kinase signaling
NCI :
LKB1 signaling events
NCI :
LPA4-mediated signaling events
NCI :
Nongenotropic Androgen signaling
NCI :
p38 signaling mediated by MAPKAP kinases
NCI :
Regulation of nuclear SMAD2/3 signaling
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Signaling mediated by p38-alpha and p38-beta
NCI :
Trk receptor signaling mediated by PI3K and PLC-gamma
NCI :
Trk receptor signaling mediated by the MAPK pathway
NCI :
Validated targets of C-MYC transcriptional repression
NCI :
VEGFR3 signaling in lymphatic endothelium
Panther :
Enkephalin release
Panther :
Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Reactome :
Activated TLR4 signalling
Reactome :
Activation of NMDA receptor upon glutamate binding and postsynaptic events
Reactome :
Adaptive Immune System
Reactome :
AKT phosphorylates targets in the nucleus
Reactome :
Axon guidance
Reactome :
Ca-dependent events
Reactome :
Calmodulin induced events
Reactome :
CaM pathway
Reactome :
CaMK IV-mediated phosphorylation of CREB
Reactome :
Circadian Clock
Reactome :
Constitutive PI3K/AKT Signaling in Cancer
Reactome :
CREB phosphorylation
Reactome :
CREB phosphorylation through the activation of Adenylate Cyclase
Reactome :
CREB phosphorylation through the activation of CaMKII
Reactome :
CREB phosphorylation through the activation of CaMKK
Reactome :
CREB phosphorylation through the activation of Ras
Reactome :
DAG and IP3 signaling
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
Downstream signaling of activated FGFR
Reactome :
EGFR interacts with phospholipase C-gamma
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
G-protein mediated events
Reactome :
GAB1 signalosome
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MAPK targets/ Nuclear events mediated by MAP kinases
Reactome :
Mitochondrial biogenesis
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
NCAM signaling for neurite out-growth
Reactome :
Neuronal System
Reactome :
Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
NOTCH2 intracellular domain regulates transcription
Reactome :
Nuclear Events (kinase and transcription factor activation)
Reactome :
Opioid Signalling
Reactome :
Organelle biogenesis and maintenance
Reactome :
Phospholipase C-mediated cascade
Reactome :
PI-3K cascade
Reactome :
PI3K events in ERBB2 signaling
Reactome :
PI3K events in ERBB4 signaling
Reactome :
PI3K/AKT activation
Reactome :
PI3K/AKT Signaling in Cancer
Reactome :
PIP3 activates AKT signaling
Reactome :
PKA-mediated phosphorylation of CREB
Reactome :
PLC beta mediated events
Reactome :
PLC-gamma1 signalling
Reactome :
PLCG1 events in ERBB2 signaling
Reactome :
Post NMDA receptor activation events
Reactome :
Role of LAT2/NTAL/LAB on calcium mobilization
Reactome :
Signal Transduction
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by NOTCH
Reactome :
Signaling by NOTCH2
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signalling by NGF
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
Transcriptional activation of mitochondrial biogenesis
Reactome :
Transmission across Chemical Synapses
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
Adipogenesis
WikiPathway :
Androgen receptor signaling pathway
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Energy Metabolism
WikiPathway :
Estrogen signaling pathway
WikiPathway :
FSH signaling pathway
WikiPathway :
G1 to S cell cycle control
WikiPathway :
Hypothetical Network for Drug Addiction
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
miRNA regulation of DNA Damage Response
WikiPathway :
miRNAs involved in DDR
WikiPathway :
Mitochondrial Gene Expression
WikiPathway :
Myometrial Relaxation and Contraction Pathways
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Serotonin HTR1 Group and FOS Pathway
WikiPathway :
Serotonin Receptor 4/6/7 and NR3C Signaling
WikiPathway :
SIDS Susceptibility Pathways
WikiPathway :
SREBP signalling
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TSH signaling pathway
Protein
SMART domain :
UniProt :
UniProt :
UniProt :
HPRD :
00442
Clone
Organism
Species Specific ID:
Genome
Reagents
Labome :