HIST1H4H (Homo sapiens) in pLenti6.2/V5-DEST

Explanation of Terms

Gene:
Gene Symbol:  HIST4H4
Gene Name:  histone cluster 4, H4
Original Clone ID:
FLH269257.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.2/V5-DEST
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00330333
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 309nts
Open reading frame: 1 to 309

ATGTCTGGCCGTGGTAAAGGTGGAAAAGGTTTGGGTAAGGGAGGAGCTAAGCGTCATCGC
AAGGTTTTGCGCGATAACATCCAGGGCATCACTAAGCCAGCTATCCGGCGCCTTGCTCGT
CGCGGCGGTGTCAAGCGAATTTCTGGCCTTATCTATGAGGAGACTCGTGGTGTTCTGAAG
GTGTTCCTGGAGAACGTGATTCGTGACGCTGTCACTTACACAGAGCACGCCAAACGCAAG
ACCGTGACAGCAATGGATGTGGTCTACGCGCTGAAGCGACAGGGACGCACTCTTTACGGC
TTCGGTGGC

Protein sequence predicted based on nucleotide sequence

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 351
Start on reference sequence 40
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable