Sputw3181_1245 (S. sp. W3-18-1 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Sputw3181_1245
Gene Name:  hybrid sensory histidine kinase BarA
Original Clone ID:
APC36647.1_SG209_pMCSG19
Keyword:
partial cds
Species:
Shewanella sp. W3-18-1
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
purifi_date: 2009-07-29 yield: imacI-82.752 and imacII-54.04
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI
Midwest Center for Structural Genomics
SsCD00330707
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 423nts
Open reading frame: 1 to 423

CATCGCTGGATCACAAGACCTAAATTCACCCATTTTGACCTGCATACGCTGAACTGGGAT
CTTTGCTTAACTCAAGCAAATCATAAATCGAACCTCGCCCTTGAGATGTTAAAAATGCTG
CTGGACTCACTACCTGAAACGGTAGAGAAAATTCAAACGGCGCTAGGCCAAAATGATCAG
GCCACTATGCTCAGTACAATCCATAAGCTGCATGGCGCAAGCTGCTATTGCGGCGTCCCC
ACCACTCAAAGGTTATGCCAAGAGATTGAATCCGCGTTAAAACGTCAAACCCCTGTGGAA
GATCTCGAACCTGAAATACTAGAGTTACTTGATGAGTTAACTAAGGTAGAATCAGCGGTA
AAACAAGTGCTATCACAGCTATCAGCGGAAATAACAGATGACCAAAAAAACGGGCTTCTT
TAA

Protein sequence predicted based on nucleotide sequence

HRWITRPKFTHFDLHTLNWDLCLTQANHKSNLALEMLKMLLDSLPETVEKIQTALGQNDQ
ATMLSTIHKLHGASCYCGVPTTQRLCQEIESALKRQTPVEDLEPEILELLDELTKVESAV
KQVLSQLSAEITDDQKNGLL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC36647.1
Recommended expression in:
Not Applicable