SPO3024 (R. pomeroyi DSS-3 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SPO3024
Gene Name:  adenylate/guanylate cyclase/hydrolase, alpha/beta fold family
Original Clone ID:
APC36678.2_SG209_pMCSG19
Keyword:
partial cds
Species:
Ruegeria pomeroyi DSS-3
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
purifi_date: 2009-08-03 yield: imacI-147.9 and imacII-81.25
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI
Midwest Center for Structural Genomics
SpCD00330736
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 564nts
Open reading frame: 1 to 564

ATGGAGCGTCGTCTCTGCGCCATTCTGGCCGCCGACATGGCAGGCTACAGCCGCCTGATG
GAGCGCAACGAAACAGATGTTCTGAACCGTCAAAAGCTCTATCGGCGCGAGCTGATCGAC
CCGGCCATCGCGCAGGCCGGCGGCCAGATCGTCAAGACCACTGGCGACGGCATGCTGGCG
CGCTTCGATACCGCGCAGGCAGCGTTGCGCTGCGCCCTGGAGATCCAACAGGCGATGCAG
CAGCGCGAGGAGGATACCCCCCGTAAGGAGCGCATCCAGTATCGTATCGGTATCAACATC
GGCGATATCGTCCTGGAAGACGGGGATATATTCGGCGACGCGGTCAATGTCGCCGCCCGG
CTGGAAGCGATTTCCGAACCCGGCGCGATCTGCGTCTCGGATATCGTGCACCAGATAACC
CAGGACCGTGTATCGGAGCCGTTCACCGATCTCGGCCTGCAAAAGGTCAAGAACATCACC
CGCCCGATCCGGGTCTGGCAATGGGTGCCCGATGCCGACCGGGACCAGAGCCACGACCCG
CAGCCCTCTCATGTCCAGCATTAA

Protein sequence predicted based on nucleotide sequence

MERRLCAILAADMAGYSRLMERNETDVLNRQKLYRRELIDPAIAQAGGQIVKTTGDGMLA
RFDTAQAALRCALEIQQAMQQREEDTPRKERIQYRIGINIGDIVLEDGDIFGDAVNVAAR
LEAISEPGAICVSDIVHQITQDRVSEPFTDLGLQKVKNITRPIRVWQWVPDADRDQSHDP
QPSHVQH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC36678.2
Recommended expression in:
Not Applicable