VC0486 (V. cholerae O1 biovar El Tor str. N16961 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VC0486
Gene Name:  DeoR family transcriptional regulator
Original Clone ID:
APC36736.2_SG211_pMCSG19
Keyword:
partial cds
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
purifi_date: yield:
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
VcCD00330827
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 477nts
Open reading frame: 1 to 477

GAAGTGATGCACGATGAATTGAATGAAAAAGTTTCAGTTCGAAAGATTTCTCTGGCTAAA
GCTGCAGCGAAACTAATCCGTGATCATAATCGGATTGTGATTGATAGTGGCAGCACCACG
GCGGCGTTAATTCAGCAACTGAATGACAAGCGAGGGCTGGTCGTGATGACCAACTCATTG
CATGTCGCGAATGCACTCAATGAATTAGAAAATGAACCCACACTGCTGATGACTGGCGGT
ACTTGGGATACCCATTCTGAATCCTTCCAAGGCAAAGTCGCAGAATCGGTACTGCGCTCT
TACGATTTTGACCAGCTGTTTATCGGTGCTGATGGCATCGATCTTGACCGGGGGACGACC
ACGTTCAATGAGCTGGTTGGGCTTAGCAAAGTGATGGCTGAAGTCTCGCGTGAAGTGATC
GTGATGATTGAGTCAGACAAAGTGGGCCGCAAAAATCCGAACTTAGAGCTGAGCTAA

Protein sequence predicted based on nucleotide sequence

EVMHDELNEKVSVRKISLAKAAAKLIRDHNRIVIDSGSTTAALIQQLNDKRGLVVMTNSL
HVANALNELENEPTLLMTGGTWDTHSESFQGKVAESVLRSYDFDQLFIGADGIDLDRGTT
TFNELVGLSKVMAEVSREVIVMIESDKVGRKNPNLELS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t455a,I152N,
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC36736.2
Recommended expression in:
Not Applicable