DNASU Plasmid | Detailed Plasmid Information

Explanation of Terms

Gene:

Gene Symbol: DVL2

Gene Name: dishevelled segment polarity protein 2

Original Clone ID:

HR4606C-417-551-14.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI
Northeast Structural Genomics Consortium

HsCD00331429

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 411nts

Open reading frame: 1 to 411

Reference Sequence Alignment

ATGCTCTCCGTCCATACGGATATGGCATCGGTGACCAAGGCCATGGCAGCTCCAGAGTCT
GGACTGGAAGTCCGGGACCGCATGTGGCTCAAGATCACCATCCCTAATGCCTTTCTGGGC
TCGGATGTGGTTGACTGGCTCTACCATCACGTGGAGGGCTTTCCTGAGCGGCGGGAGGCC
CGCAAGTATGCCAGCGGGCTGCTCAAAGCAGGCCTGATCCGACACACCGTCAACAAGATC
ACCTTCTCTGAGCAGTGCTATTACGTCTTCGGAGACCTCAGTGGTGGCTGTGAGAGCTAC
CTAGTCAACCTGTCTCTCAATGACAACGATGGCTCCAGTGGGGCTTCAGACCAGGATACC
CTGGCTCCTCTGCCTGGGGCCACCCCCTGGCCCCTGCTGCCCACTTTCTAG

Protein sequence predicted based on nucleotide sequence

MLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREA
RKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDT
LAPLPGATPWPLLPTF*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c21t,D7D,

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR4606C

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

DVL2

Symbol Nomenclature :

DVL2

Designation :

dishevelled 2 (homologous to Drosophila dsh)|dishevelled, dsh homolog 2|segment polarity protein dishevelled homolog DVL-2

Full Nomenclature :

dishevelled segment polarity protein 2

GENEID :

1856

HGNC :

3086

MIM :

602151

Vega :

OTTHUMG00000102155

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      ATGGCGGGTAGCAGCACTGGGGGCGGTGGGGTTGGGGAGACGAAGGTGATTTACCACCTG
NM_004422.2         ATGGCGGGTAGCAGCACTGGGGGCGGTGGGGTTGGGGAGACGAAGGTGATTTACCACCTG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GATGAGGAAGAGACTCCCTACCTGGTGAAGATCCCTGTCCCCGCCGAGCGCATCACCCTC
NM_004422.2         GATGAGGAAGAGACTCCCTACCTGGTGAAGATCCCTGTCCCCGCCGAGCGCATCACCCTC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GGCGATTTCAAGAGCGTCCTGCAGCGGCCCGCGGGCGCCAAGTACTTTTTCAAGTCTATG
NM_004422.2         GGCGATTTCAAGAGCGTCCTGCAGCGGCCCGCGGGCGCCAAGTACTTTTTCAAGTCTATG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GATCAGGATTTCGGGGTGGTGAAGGAAGAAATTTCAGATGACAACGCCCGCCTCCCCTGC
NM_004422.2         GATCAGGATTTCGGGGTGGTGAAGGAAGAAATTTCAGATGACAACGCCCGCCTCCCCTGC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      TTCAACGGAAGGGTGGTATCCTGGCTGGTGTCCTCAGATAATCCCCAACCCGAGATGGCC
NM_004422.2         TTCAACGGAAGGGTGGTATCCTGGCTGGTGTCCTCAGATAATCCCCAACCCGAGATGGCC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      CCTCCAGTCCATGAGCCTCGGGCAGAACTGGCGCCTCCAGCCCCACCTTTACCTCCTTTG
NM_004422.2         CCTCCAGTCCATGAGCCTCGGGCAGAACTGGCGCCTCCAGCCCCACCTTTACCTCCTTTG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      CCACCCGAGAGGACCAGCGGCATTGGGGACTCAAGGCCTCCATCCTTCCACCCTAATGTG
NM_004422.2         CCACCCGAGAGGACCAGCGGCATTGGGGACTCAAGGCCTCCATCCTTCCACCCTAATGTG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      TCCAGCAGCCATGAGAATCTGGAGCCTGAGACAGAAACCGAGTCAGTAGTGTCACTGAGG
NM_004422.2         TCCAGCAGCCATGAGAATCTGGAGCCTGAGACAGAAACCGAGTCAGTAGTGTCACTGAGG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      CGGGAGCGGCCTCGCAGGAGAGACAGCACTGG------------GGGCCACAGGACTGGT
NM_004422.2         CGGGAGCGGCCTCGCAGGAGAGACAGCAGTGAGCATGGCGCTGGGGGCCACAGGACTGGT
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GGCCCCTCAAGGCTGGAGCGCCACCTGGCCGGATACGAGAGCTCCTCTACCCTCATGACC
NM_004422.2         GGCCCCTCAAGGCTGGAGCGCCACCTGGCCGGATACGAGAGCTCCTCTACCCTCATGACC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      AGCGAGCTGGAGAGTACCAGCCTGGGGGACTCGGACGAGGAGGACACCATGAGCAGGTTC
NM_004422.2         AGCGAGCTGGAGAGTACCAGCCTGGGGGACTCGGACGAGGAGGACACCATGAGCAGGTTC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      AGCAGCTCCACGGAGCAGAGCAGTGCCTCCCGCCTCCTTAAGCGCCACCGGCGGCGAAGG
NM_004422.2         AGCAGCTCCACGGAGCAGAGCAGTGCCTCCCGCCTCCTTAAGCGCCACCGGCGGCGAAGG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      AAGCAGAGGCCACCCCGCCTGGAGAGGACGTCATCCTTCAGCAGCGTCACAGATTCCACA
NM_004422.2         AAGCAGAGGCCACCCCGCCTGGAGAGGACGTCATCCTTCAGCAGCGTCACAGATTCCACA
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      ATGTCTCTCAATATCATCACAGTCACGCTAAACATGGAGAAGTACAACTTCCTGGGTATC
NM_004422.2         ATGTCTCTCAATATCATCACAGTCACGCTAAACATGGAGAAGTACAACTTCCTGGGTATC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      TCCATTGTTGGCCAGAGCAATGAGCGGGGAGACGGAGGCATCTACATTGGCTCCATCATG
NM_004422.2         TCCATTGTTGGCCAGAGCAATGAGCGGGGAGACGGAGGCATCTACATTGGCTCCATCATG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      AAGGGTGGGGCTGTGGCGGCCGACGGGCGCATTGAGCCAGGGGACATGCTTTTGCAGGTG
NM_004422.2         AAGGGTGGGGCTGTGGCGGCCGACGGGCGCATTGAGCCAGGGGACATGCTTTTGCAGGTG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      AATGACATGAACTTTGAGAACATGAGCAACGATGACGCTGTGCGGGTGCTGAGGGACATT
NM_004422.2         AATGACATGAACTTTGAGAACATGAGCAACGATGACGCTGTGCGGGTGCTGAGGGACATT
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GTGCACAAGCCTGGCCCCATTGTGCTGACTGTGGCCAAGTGCTGGGATCCCTCTCCTCAG
NM_004422.2         GTGCACAAGCCTGGCCCCATTGTGCTGACTGTGGCCAAGTGCTGGGATCCCTCTCCTCAG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GCCTATTTCACTCTCCCCCGAAATGAGCCCATCCAGCCAATTGACCCTGCTGCCTGGGTG
NM_004422.2         GCCTATTTCACTCTCCCCCGAAATGAGCCCATCCAGCCAATTGACCCTGCTGCCTGGGTG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      TCCCATTCCGCGGCTCTGACTGGCACCTTCCCAGCCTATCCAGGTTCCTCCTCCATGAGC
NM_004422.2         TCCCATTCCGCGGCTCTGACTGGCACCTTCCCAGCCTATCCAGGTTCCTCCTCCATGAGC
                                                                                

HsCD00331429        ---------------------------------------------ATGCTCTCCGTCCAT
XM_005256502.1      ACCATTACATCTGGATCGTCTTTGCCTGATGGCTGTGAAGGCCGGGGTCTCTCCGTCCAT
NM_004422.2         ACCATTACATCTGGATCGTCTTTGCCTGATGGCTGTGAAGGCCGGGGTCTCTCCGTCCAT
                                                                 .  ************

HsCD00331429        ACGGATATGGCATCGGTGACCAAGGCCATGGCAGCTCCAGAGTCTGGACTGGAAGTCCGG
XM_005256502.1      ACGGACATGGCATCGGTGACCAAGGCCATGGCAGCTCCAGAGTCTGGACTGGAAGTCCGG
NM_004422.2         ACGGACATGGCATCGGTGACCAAGGCCATGGCAGCTCCAGAGTCTGGACTGGAAGTCCGG
                    ***** ******************************************************

HsCD00331429        GACCGCATGTGGCTCAAGATCACCATCCCTAATGCCTTTCTGGGCTCGGATGTGGTTGAC
XM_005256502.1      GACCGCATGTGGCTCAAGATCACCATCCCTAATGCCTTTCTGGGCTCGGATGTGGTTGAC
NM_004422.2         GACCGCATGTGGCTCAAGATCACCATCCCTAATGCCTTTCTGGGCTCGGATGTGGTTGAC
                    ************************************************************

HsCD00331429        TGGCTCTACCATCACGTGGAGGGCTTTCCTGAGCGGCGGGAGGCCCGCAAGTATGCCAGC
XM_005256502.1      TGGCTCTACCATCACGTGGAGGGCTTTCCTGAGCGGCGGGAGGCCCGCAAGTATGCCAGC
NM_004422.2         TGGCTCTACCATCACGTGGAGGGCTTTCCTGAGCGGCGGGAGGCCCGCAAGTATGCCAGC
                    ************************************************************

HsCD00331429        GGGCTGCTCAAAGCAGGCCTGATCCGACACACCGTCAACAAGATCACCTTCTCTGAGCAG
XM_005256502.1      GGGCTGCTCAAAGCAGGCCTGATCCGACACACCGTCAACAAGATCACCTTCTCTGAGCAG
NM_004422.2         GGGCTGCTCAAAGCAGGCCTGATCCGACACACCGTCAACAAGATCACCTTCTCTGAGCAG
                    ************************************************************

HsCD00331429        TGCTATTACGTCTTCGGAGACCTCAGTGGTGGCTGTGAGAGCTACCTAGTCAACCTGTCT
XM_005256502.1      TGCTATTACGTCTTCGGAGACCTCAGTGGTGGCTGTGAGAGCTACCTAGTCAACCTGTCT
NM_004422.2         TGCTATTACGTCTTCGGAGACCTCAGTGGTGGCTGTGAGAGCTACCTAGTCAACCTGTCT
                    ************************************************************

HsCD00331429        CTCAATGACAACGATGGCTCCAGTGGGGCTTCAGACCAGGATACCCTGGCTCCTCTGCCT
XM_005256502.1      CTCAATGACAACGATGGCTCCAGTGGGGCTTCAGACCAGGATACCCTGGCTCCTCTGCCT
NM_004422.2         CTCAATGACAACGATGGCTCCAGTGGGGCTTCAGACCAGGATACCCTGGCTCCTCTGCCT
                    ************************************************************

HsCD00331429        GGGGCCACCCCCTGGCCCCTGCTGCCCACTTTCTAG------------------------
XM_005256502.1      GGGGCCACCCCCTGGCCCCTGCTGCCCACTTTCTCCTACCAATACCCTGCCCCACACCCC
NM_004422.2         GGGGCCACCCCCTGGCCCCTGCTGCCCACTTTCTCCTACCAATACCCTGCCCCACACCCC
                    **********************************.                         

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      TACAGCCCGCAGCCTCCACCCTACCATGAGCTTTCATCTTACACCTATGGTGGGGGCAGT
NM_004422.2         TACAGCCCGCAGCCTCCACCCTACCATGAGCTTTCATCTTACACCTATGGTGGGGGCAGT
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GCCAGCAGCCAGCATAGTGAGGGCAGCCGGAGCAGTGGGTCGACACGGAGTGATGGGGGG
NM_004422.2         GCCAGCAGCCAGCATAGTGAGGGCAGCCGGAGCAGTGGGTCGACACGGAGTGATGGGGGG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GCAGGGCGCACGGGGAGGCCCGAGGAGCGGGCCCCCGAGTCCAAGTCCGGCAGTGGCAGT
NM_004422.2         GCAGGGCGCACGGGGAGGCCCGAGGAGCGGGCCCCCGAGTCCAAGTCCGGCAGTGGCAGT
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GAGTCTGAGCCCTCCAGCCGAGGGGGCAGCCTTCGGCGGGGTGGGGAAGCAAGTGGGACT
NM_004422.2         GAGTCTGAGCCCTCCAGCCGAGGGGGCAGCCTTCGGCGGGGTGGGGAAGCAAGTGGGACT
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      AGCGATGGGGGCCCTCCTCCATCCAGAGGCTCAACTGGGGGTGCCCCTAATCTCCGAGCC
NM_004422.2         AGCGATGGGGGCCCTCCTCCATCCAGAGGCTCAACTGGGGGTGCCCCTAATCTCCGAGCC
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      CACCCAGGGCTCCATCCCTATGGACCGCCCCCTGGCATGGCCCTCCCCTACAACCCCATG
NM_004422.2         CACCCAGGGCTCCATCCCTATGGACCGCCCCCTGGCATGGCCCTCCCCTACAACCCCATG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      ATGGTGGTCATGATGCCCCCACCTCCACCTCCAGTCCCTCCAGCAGTGCAGCCTCCGGGG
NM_004422.2         ATGGTGGTCATGATGCCCCCACCTCCACCTCCAGTCCCTCCAGCAGTGCAGCCTCCGGGG
                                                                                

HsCD00331429        ------------------------------------------------------------
XM_005256502.1      GCCCCTCCAGTCAGAGACCTGGGCTCTGTGCCCCCAGAACTGACAGCCAGCCGCCAAAGC
NM_004422.2         GCCCCTCCAGTCAGAGACCTGGGCTCTGTGCCCCCAGAACTGACAGCCAGCCGCCAAAGC
                                                                                

HsCD00331429        ---------------------------------------------------
XM_005256502.1      TTCCACATGGCCATGGGCAATCCCAGCGAGTTCTTTGTGGATGTTATGTAG
NM_004422.2         TTCCACATGGCCATGGGCAATCCCAGCGAGTTCTTTGTGGATGTTATGTAG