Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol:  BRAF
Gene Name:  v-raf murine sarcoma viral oncogene homolog B
Original Clone ID:
HR4694F-149-232-14.5
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
HsCD00331794
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 258nts
Open reading frame: 1 to 258

ATGCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA
GTGGCACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG
AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA
ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGTTG
GAGAATGTTCCACTTTAG

Protein sequence predicted based on nucleotide sequence

MPKSPQKPIVRVFLPNKQRTVAPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKP
IGWDTDISWLTGEELHVEVLENVPL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t65c,V22A,
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in HR4694F
Recommended expression in:
Not Applicable