DNASU Plasmid | Detailed Plasmid Information

Explanation of Terms

Gene:

Gene Symbol: MAP3K10

Gene Name: mitogen-activated protein kinase kinase kinase 10

Original Clone ID:

HR4517D-64-369-14.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Northeast Structural Genomics Consortium

HsCD00331846

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 924nts

Open reading frame: 1 to 924

Reference Sequence Alignment

ATGCCCAGCGGCCGCGTGGGCGTCTTCCCCAGCAACTACGTGGCCCCCGGCGCCCCCGCT
GCACCCGCGGGCCTCCAGCTGCCCCAGGAGATCCCCTTCCACGAGCTGCAGCTAGAGGAG
ATCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTGGCGTGGCGAGGAGGTG
GCAGTCAAGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAGCAGGTG
TGCCAGGAAGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTTAGGGGC
GCCTGCCTCAACCCCCCACACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGTGCACTG
AGCAGGGTGCTGGCAGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCTGTGCAG
GTGGCCCGGGGCATGAACTACCTACACAATGATGCCCCTGTGCCCATCATCCACCGGGAC
CTCAAGTCCATCAACATCCTGATCCTGGAGGCCATCGAGAACCACAACCTCGCAGACACG
GTGCTCAAGATCACGGACTTCGGCCTCGCCCGCGAGTGGCACAAGACCACCAAGATGAGC
GCTGCGGGGACCTACGCCTGGATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTCTCCAAA
AGCAGTGATGTCTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAGGTCCCC
TACCGTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAGCTGACGCTG
CCCATTCCCTCCACGTGCCCCGAGCCCTTTGCCCGCCTCCTGGAGGAATGCTGGGACCCA
GACCCCCACGGGCGGCCAGATTTCGGTAGCATCTTGAAGCGGCTTGAAGTCATCGAACAG
TCAGCCCTGTTCCAGATGCCATAG

Protein sequence predicted based on nucleotide sequence

MPSGRVGVFPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEV
AVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGAL
SRVLAGRRVPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLADT
VLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVP
YREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDFGSILKRLEVIEQ
SALFQMP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR4517D

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

MAP3K10

Symbol Nomenclature :

MAP3K10

SYNONYM :

MEKK10; MLK2; MST

Designation :

MKN28 derived nonreceptor_type serine/threonine kinase|MKN28 kinase|mixed lineage kinase 2|protein kinase MST

Full Nomenclature :

mitogen-activated protein kinase kinase kinase 10

GENEID :

4294

HGNC :

6849

MIM :

600137

Vega :

OTTHUMG00000182591

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00331846      ------------------------------------------------------------
NM_002446.3       ATGGAGGAGGAGGAGGGGGCGGTGGCCAAGGAGTGGGGCACGACCCCCGCGGGGCCCGTC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       TGGACCGCGGTGTTCGACTACGAGGCGGCGGGCGACGAGGAGCTGACCCTGCGGAGGGGC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GATCGCGTCCAGGTGCTTTCCCAAGACTGTGCGGTGTCCGGCGACGAGGGCTGGTGGACC
                                                                              

HsCD00331846      ------ATGCCCAGCGGCCGCGTGGGCGTCTTCCCCAGCAACTACGTGGCCCCCGGCGCC
NM_002446.3       GGGCAGCTCCCCAGCGGCCGCGTGGGCGTCTTCCCCAGCAACTACGTGGCCCCCGGCGCC
                        .* ***************************************************

HsCD00331846      CCCGCTGCACCCGCGGGCCTCCAGCTGCCCCAGGAGATCCCCTTCCACGAGCTGCAGCTA
NM_002446.3       CCCGCTGCACCCGCGGGCCTCCAGCTGCCCCAGGAGATCCCCTTCCACGAGCTGCAGCTA
                  ************************************************************

HsCD00331846      GAGGAGATCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTGGCGTGGCGAG
NM_002446.3       GAGGAGATCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTGGCGTGGCGAG
                  ************************************************************

HsCD00331846      GAGGTGGCAGTCAAGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAG
NM_002446.3       GAGGTGGCAGTCAAGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAG
                  ************************************************************

HsCD00331846      CAGGTGTGCCAGGAAGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTT
NM_002446.3       CAGGTGTGCCAGGAAGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTT
                  ************************************************************

HsCD00331846      AGGGGCGCCTGCCTCAACCCCCCACACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGT
NM_002446.3       AGGGGCGCCTGCCTCAACCCCCCACACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGT
                  ************************************************************

HsCD00331846      GCACTGAGCAGGGTGCTGGCAGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCT
NM_002446.3       GCACTGAGCAGGGTGCTGGCAGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCT
                  ************************************************************

HsCD00331846      GTGCAGGTGGCCCGGGGCATGAACTACCTACACAATGATGCCCCTGTGCCCATCATCCAC
NM_002446.3       GTGCAGGTGGCCCGGGGCATGAACTACCTACACAATGATGCCCCTGTGCCCATCATCCAC
                  ************************************************************

HsCD00331846      CGGGACCTCAAGTCCATCAACATCCTGATCCTGGAGGCCATCGAGAACCACAACCTCGCA
NM_002446.3       CGGGACCTCAAGTCCATCAACATCCTGATCCTGGAGGCCATCGAGAACCACAACCTCGCA
                  ************************************************************

HsCD00331846      GACACGGTGCTCAAGATCACGGACTTCGGCCTCGCCCGCGAGTGGCACAAGACCACCAAG
NM_002446.3       GACACGGTGCTCAAGATCACGGACTTCGGCCTCGCCCGCGAGTGGCACAAGACCACCAAG
                  ************************************************************

HsCD00331846      ATGAGCGCTGCGGGGACCTACGCCTGGATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTC
NM_002446.3       ATGAGCGCTGCGGGGACCTACGCCTGGATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTC
                  ************************************************************

HsCD00331846      TCCAAAAGCAGTGATGTCTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAG
NM_002446.3       TCCAAAAGCAGTGATGTCTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAG
                  ************************************************************

HsCD00331846      GTCCCCTACCGTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAGCTG
NM_002446.3       GTCCCCTACCGTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAGCTG
                  ************************************************************

HsCD00331846      ACGCTGCCCATTCCCTCCACGTGCCCCGAGCCCTTTGCCCGCCTCCTGGAGGAATGCTGG
NM_002446.3       ACGCTGCCCATTCCCTCCACGTGCCCCGAGCCCTTTGCCCGCCTCCTGGAGGAATGCTGG
                  ************************************************************

HsCD00331846      GACCCAGACCCCCACGGGCGGCCAGATTTCGGTAGCATCTTGAAGCGGCTTGAAGTCATC
NM_002446.3       GACCCAGACCCCCACGGGCGGCCAGATTTCGGTAGCATCTTGAAGCGGCTTGAAGTCATC
                  ************************************************************

HsCD00331846      GAACAGTCAGCCCTGTTCCAGATGCCATAG------------------------------
NM_002446.3       GAACAGTCAGCCCTGTTCCAGATGCCACTGGAGTCCTTCCACTCGCTGCAGGAAGACTGG
                  *************************** :*                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       AAGCTGGAGATTCAGCACATGTTTGATGACCTTCGGACCAAGGAGAAGGAGCTTCGGAGC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CGTGAGGAGGAGCTGCTGCGGGCGGCACAGGAGCAGCGCTTCCAGGAGGAGCAGCTGCGG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CGGCGGGAGCAGGAGCTGGCAGAACGTGAGATGGACATCGTGGAACGGGAGCTGCACCTG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CTCATGTGCCAGCTGAGCCAGGAGAAGCCCCGGGTCCGCAAGCGCAAGGGCAACTTCAAG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CGCAGCCGCCTGCTCAAGCTGCGGGAAGGCGGCAGCCACATCAGCCTGCCCTCTGGCTTT
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GAGCATAAGATCACAGTCCAGGCCTCTCCAACTCTGGATAAGCGGAAAGGATCCGATGGG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GCCAGCCCCCCTGCAAGCCCCAGCATCATCCCCCGGCTGAGGGCCATTCGCCTGACTCCC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GTGGACTGTGGTGGCAGCAGCAGTGGCAGCAGCAGTGGAGGAAGTGGGACATGGAGCCGC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GGTGGGCCCCCAAAGAAGGAAGAACTGGTCGGGGGCAAGAAGAAGGGACGAACGTGGGGG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CCCAGCTCCACCCTGCAGAAGGAGCGGGTGGGAGGAGAGGAGAGGCTGAAGGGGCTGGGG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GAAGGAAGCAAACAGTGGTCATCAAGTGCCCCCAACCTGGGCAAGTCCCCCAAACACACA
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CCCATCGCCCCTGGCTTTGCCAGCCTCAATGAGATGGAGGAGTTCGCGGAGGCAGAGGAT
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GGAGGCAGCAGCGTGCCCCCTTCCCCCTACTCGACCCCGTCCTACCTCTCAGTGCCACTG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CCTGCCGAGCCCTCCCCGGGGGCGCGGGCGCCGTGGGAGCCGACGCCGTCCGCGCCCCCC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GCTCGGTGGGGACACGGCGCCCGGCGGCGCTGCGACCTGGCGCTGCTAGGCTGCGCCACG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CTGCTGGGGGCTGTGGGCCTGGGCGCCGACGTGGCCGAGGCGCGCGCGGCCGACGGTGAG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GAGCAGCGGCGCTGGCTCGACGGCCTCTTCTTTCCCCGCGCCGGCCGCTTCCCGCGGGGC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CTCAGCCCACCCGCGCGTCCCCACGGCCGCCGCGAAGACGTGGGCCCCGGCCTGGGCCTG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GCGCCCTCGGCCACCCTCGTGTCGCTGTCGTCCGTGTCCGACTGCAACTCCACGCGTTCA
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CTGCTGCGCTCTGACAGTGACGAGGCCGCACCGGCCGCGCCCTCCCCACCACCCTCCCCG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CCCGCGCCCACACCCACGCCCTCGCCCAGCACCAACCCCCTGGTGGACCTGGAGCTGGAG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       AGCTTCAAGAAGGACCCCCGCCAGTCGCTCACGCCCACCCACGTCACGGCTGCATGCGCT
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GTGAGCCGCGGGCACCGGCGGACGCCATCGGACGGGGCGCTGGGGCAGCGGGGGCCGCCC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       GAGCCCGCGGGCCATGGCCCTGGCCCTCGTGACCTTCTGGACTTCCCCCGCCTGCCCGAC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CCCCAGGCCCTGTTCCCAGCCCGCCGCCGGCCCCCTGAGTTCCCAGGCCGCCCCACCACC
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CTGACCTTTGCCCCGAGACCTCGGCCGGCTGCCAGTCGCCCCCGCTTGGACCCCTGGAAA
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       CTGGTCTCCTTCGGCCGGACACTCACCATCTCGCCTCCCAGCAGGCCAGACACTCCGGAG
                                                                              

HsCD00331846      ------------------------------------------------------------
NM_002446.3       AGCCCTGGGCCCCCCAGCGTGCAGCCCACACTGCTGGACATGGACATGGAGGGGCAGAAC
                                                                              

HsCD00331846      ---------------------------------------------
NM_002446.3       CAAGACAGCACAGTGCCCCTGTGCGGGGCCCACGGCTCCCACTAA