SPO1173 (R. pomeroyi DSS-3 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SPO1173
Gene Name:  diguanylate cyclase, putative
Original Clone ID:
67658
Keyword:
SP or TM removed
Species:
Ruegeria pomeroyi DSS-3
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SpCD00332278
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1143nts
Open reading frame: 1 to 1143

ACCAACCGCGCCCGTTTCGCGGCACTGCAATCGCGATCCGAAACCGAACGCACGTTGATG
GACGAACAATTGCGGCTGGCGCGCGAGGTGCGGCTGCTGGGCGAGTTGAACGAATGGCTG
CAATCCTGCCGCTCGCTGGAGGAGCTGTTCGCGATGGTGTCGCGCTTCATGGAACATATC
CTGCCCGATGCCGAGGGCAGTCTCTATGTCTATTCCAACTCGCGTGATGTGCTGGATGGC
AGCGCCGGTTGGAATGGCGGGGCGTTCAAGGATCACATCCACCCCGAGGCCTGCTGGGGC
CTGCGCCGCGGGCGCTCTTATGAATATGGCGCATCCGAGGTGGATTTCGTCTGCGACCAT
GCCGAACCGCATGATGGCCGGCCCTATTTCTGCTTCCCGGTGTTGGCGCATGGCGAAACC
GTCGGCCTGATGCATCTGCGCGCCCAGCCCGGGGCCGAGGCGGAATTCTCCGCCAGCAAG
CGGCTGGCGCAGATGTGTGCCGAACAGATCAGCATGGCGATCGCCAATGTGCGGATGCGC
GACCAGTTGCACGACCAGTCGGTGCGTGACCCGCTGACCGGCCTGTTCAACCGGCGCCAC
CTGACCGAAACCCTGCGCAAGCAGTTGCTGCATTGCCAGACGCGCGGCGGCCGGCTCAGC
CTGATCGCGGTCGATGTCGATCACTTCAAGAAATTCAACGACACCCACGGCCATGATGCC
GGCGACATGGTGCTACGCGCGGTCGGCGCGGCGTTGGAGCAGGCCTGTGACCGCGACGAG
GTGGCCTGTCGCATCGGCGGCGAGGAATTCATGCTGGTGCTGCTCGACTGCGCGCCCGAG
GATGCGCTGACCCGCGCCGAATTGCTGCGCCAGTCGGTCGAAGAGGTGAGTGTCCGCTAT
GGTGAAAAGACCTTGCCGCGGGTGACCATTTCGGCCGGGGTGGCGCATTACCCGCGCCAC
GGCACCATGCCCCAGGACCTGATGCGCGCCGCCGATGACGCGCTTTACGAGGCCAAGGGA
CTGGGCCGCAACCAGGTGCGCGTCGCACAGTCGCCGGGAACGGACCCGCGCGAGGCGCAG
GCCGCCAAACCGGCAAAAGCAAAGGACCCCAAGACGCCCGAAGGGAAATTCGCCGCCGAA
TAA

Protein sequence predicted based on nucleotide sequence

TNRARFAALQSRSETERTLMDEQLRLAREVRLLGELNEWLQSCRSLEELFAMVSRFMEHI
LPDAEGSLYVYSNSRDVLDGSAGWNGGAFKDHIHPEACWGLRRGRSYEYGASEVDFVCDH
AEPHDGRPYFCFPVLAHGETVGLMHLRAQPGAEAEFSASKRLAQMCAEQISMAIANVRMR
DQLHDQSVRDPLTGLFNRRHLTETLRKQLLHCQTRGGRLSLIAVDVDHFKKFNDTHGHDA
GDMVLRAVGAALEQACDRDEVACRIGGEEFMLVLLDCAPEDALTRAELLRQSVEEVSVRY
GEKTLPRVTISAGVAHYPRHGTMPQDLMRAADDALYEAKGLGRNQVRVAQSPGTDPREAQ
AAKPAKAKDPKTPEGKFAAE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 405513
Recommended expression in:
Not Applicable