BT_2192 (B. thetaiotaomicron VPI-5482 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_2192
Gene Name:  hypothetical protein
Original Clone ID:
44746
Keyword:
SP or TM removed
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
BtCD00332637
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1395nts
Open reading frame: 1 to 1395

GCTCAACAAGCACCAGCTCCTTGCGGGTTGGTTCCGAGTGCCCGTCAACTTGAATGGTAC
AATCGGGAGATGATTGCTTTCTTTCATTTTGGAATCAATACATTTGAAGAATATGTGAAT
GAAGGTGACGGGAAGGCAAGTACGGCAATTTTCAATCCCACAGCCCTGGATTGCAGGCAG
TGGATGCAGACATTAAAAGCAGCGGGGATTCCCGCTGCTATTCTGACTGCCAAGCATGCC
GACGGATTTTGCTTGTGGCCTAGCAAGTACACAGATTATAGTGTGAAGAATGCAGCGTGG
AAGAACGGTAAAGGAGATGTAGTCCGCGAGTTTGTCGATGCTTGTGAAGAGTATGGATTG
AAGGCAGGAATCTATCTGGGACCTCATGACCGTCACGAACACCTTTCACCTTTATATACG
ACCGAACGCTACAAGGAATATTATGCCCACCAGCTTGGCGAACTGATGAGTGATTACGGT
AAGATATGGGAAACATGGTGGGACGGCGCGGGAGCCGACGAATTGACAACGCCTGTTTAT
CGTCATTGGTATAAGATTGTCCGTGAAAAACAGCCGGATTGCGTTATCTTCGGTACAAAG
AATTCTTATCCGTTTGCTGATGTCCGCTGGATGGGGAATGAAGCGGGAGAAGCGGGTGAT
CCTTGTTGGGCGACGACTGACTCAGTAGCTATTCGTGATGAAGCTCAGTATTACAAAGGT
TTGAATGAGGGTATGCTGGATGGTGATGCGTATATTCCGGCAGAAACAGATGTTTCAATA
CGTCCCAGCTGGTTCTATCATGCCGAAGAGGATAGCCGTGTGAAGAGTGTCCGTGAACTT
TGGGATATCTACTGTACCTCCGTGGGGCGTAATAGTGTATTGCTTCTGAATTTCCCGCCC
GACAGACGTGGTTTGATTCATTCGACCGATTCGCTGCACGCCGCCTTGTTAAAACAGGGG
ATTGATGAGACTTTCAGTACGAATCTGTTGAGAGGAGCCAAAGTGAAAGCGACCAATGTA
CGGGGTGCAAAGTATAGTCCGGAAAAAATGCTGGACAATGAGAAAAACACTTATTTTGCC
GGAAAAGACGGAGAGGTAAAAGCGGATATAATTTTTACCTTGCCCAAAACTATTGAGTTT
GATTGTCTGATGATCGAAGAAGTTATCGAATTGGGACATCGGACGACGAAGTGGAGTGTG
GAGTACACTGTTGATGGCAAAAACTGGATCACCATTCCGGAGGCGACGGACAAGCAGGCC
ATTGGTCATAAATGGATTGTACGTCTGGCACCGGTGAAAGCAAAGCAGGTACGTTTGCGT
ATTCAGGACGGTAAGGCTTGTCCGGCTATCCATACTTTTGGCGTATATAAACAGTCACCG
GTTTTCAAATTATAA

Protein sequence predicted based on nucleotide sequence

AQQAPAPCGLVPSARQLEWYNREMIAFFHFGINTFEEYVNEGDGKASTAIFNPTALDCRQ
WMQTLKAAGIPAAILTAKHADGFCLWPSKYTDYSVKNAAWKNGKGDVVREFVDACEEYGL
KAGIYLGPHDRHEHLSPLYTTERYKEYYAHQLGELMSDYGKIWETWWDGAGADELTTPVY
RHWYKIVREKQPDCVIFGTKNSYPFADVRWMGNEAGEAGDPCWATTDSVAIRDEAQYYKG
LNEGMLDGDAYIPAETDVSIRPSWFYHAEEDSRVKSVRELWDIYCTSVGRNSVLLLNFPP
DRRGLIHSTDSLHAALLKQGIDETFSTNLLRGAKVKATNVRGAKYSPEKMLDNEKNTYFA
GKDGEVKADIIFTLPKTIEFDCLMIEEVIELGHRTTKWSVEYTVDGKNWITIPEATDKQA
IGHKWIVRLAPVKAKQVRLRIQDGKACPAIHTFGVYKQSPVFKL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in 393042
Recommended expression in:
Not Applicable