Nmul_A1332 (N. multiformis ATCC 25196 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Nmul_A1332
Gene Name:  diguanylate phosphodiesterase
Original Clone ID:
47985
Keyword:
full-length cds
Species:
Nitrosospira multiformis ATCC 25196
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
NmCD00332984
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1275nts
Open reading frame: 1 to 1275

ATGATTCTAGATACGGCTCCACATATTTCTTCGGTCGGGCAGGTAAAGGAATTCTTCCTG
GCCCGTCAACCTATTCTGGATCAGAACCAGAATCTGATCGCTTACGAGTTATTGTTCCGC
CGCGCAGGAGTCAGGGCACCGGTTAGCGCGGAGGATACCCGGGGTGCCGCTTCCATCATC
GCACATACATCCGAACTGGGCATCGAGAATGTGACCGGGAGCGCGCTCGGCTTCTTCAAC
GTGAATTCCACGCTGTTGATGGGAGACCTGGTTAATTTTCTACCCGCCGAAAAAGTTGTG
TTCGAGTTTCCTCAAACGGACAAGATTACTCCCGAACTCGTCCGGCGTGTTGCCGAACTG
GCGAAGGCAGGATATCGATTTGCGTTGGATGACGTTATCACCCTGTCCGAATACATAAAA
CCGTTACTGCCGTTTGTTGAAATCATCAAGATCGACACGTCCGTCATGACGTATGCCGAT
CTGGCCATGCTCGTTCGCCAGTTCAAACAGGCACGCAAGGTGCTTCTTGCTGACAAAGTA
GAGACGCTCGAACAATTCCAGAATTGCCATGAGCTCGGCTTTGACTATTACCAGGGTTAC
TACTTTGCAAAACCAGCTCTTCTGTCAGGCAAGAAGCTTGCAACTTCGCAACTGGCGATC
ATGGAGCTGATGACTCTGGTAGCCAGGGACGCCGACACGATAGAAATCGAGCAACGCATC
AAAAAAGAAGTATCGCTTTGCCTGTCCTTATTACGATTGGTCAATACGCCCGCCATGGGT
GTTACGAAAAGCATACATTCGTTAAGTGAGGCCTTGATGGTCCTCGGCCGCCGCCAGTTG
CAGCGATGGTTGCAAATTCAGATCTATGTCGGGCCGGACCCTCAGATCCAATTCGGGTCG
CCGCTTCTCCTGCTGGCAGCTACACGCGGCAAACTTCTTGAACTGGTTGCTCAAAAACTT
CAGCCCGCAAACAGAAGCATGGCCGATATTGCCTTTACCGTGGGCATTTTATCCTTGCTG
GATGCTTTGTTCGGGCAACACATGGAAAGAATTCTCCGCCAGATCGTTCTGAGCGAGGAG
ATCACCGAAGCCTTGCTGTACCGGACGGGGTTTTACGGGGACTTGCTGAAGCTTGCCGAA
TATCTCGAAAAACCTGGCGAAGCCGGCCGACTGCTCGTGCCCCTGCTTGCCAGATTGGGG
CTCCCGATTGAAGAGCTTTATCCATTCCAATTGGAAGCATTTGAATGGAGCAACAGTTTG
TTACCTACCTTATAA

Protein sequence predicted based on nucleotide sequence

MILDTAPHISSVGQVKEFFLARQPILDQNQNLIAYELLFRRAGVRAPVSAEDTRGAASII
AHTSELGIENVTGSALGFFNVNSTLLMGDLVNFLPAEKVVFEFPQTDKITPELVRRVAEL
AKAGYRFALDDVITLSEYIKPLLPFVEIIKIDTSVMTYADLAMLVRQFKQARKVLLADKV
ETLEQFQNCHELGFDYYQGYYFAKPALLSGKKLATSQLAIMELMTLVARDADTIEIEQRI
KKEVSLCLSLLRLVNTPAMGVTKSIHSLSEALMVLGRRQLQRWLQIQIYVGPDPQIQFGS
PLLLLAATRGKLLELVAQKLQPANRSMADIAFTVGILSLLDALFGQHMERILRQIVLSEE
ITEALLYRTGFYGDLLKLAEYLEKPGEAGRLLVPLLARLGLPIEELYPFQLEAFEWSNSL
LPTL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 394141
Recommended expression in:
Not Applicable