Sfri_0005 (S. frigidimarina NCIMB 400 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
48092
Keyword:
full-length cds
Species:
Shewanella frigidimarina NCIMB 400
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SfCD00333012
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1053nts
Open reading frame: 1 to 1053

ATGGTTATTGGTTTCTTTAAAAAATTGTTTAATGTTCGTGACCGCAATGAACAGGCTAAA
TTCACTGGCATTACCACCGCAGAAAAACGTCAACAAACCAAACCACAGACACCTGTACCT
GCAATTGAGGTTACGGAGCATCAAGCTGTTGAATCACAATTAGATCTATCGGCTTTATTT
TATAGTTTATTGTTTGCAACTCCATCCACTACGACCACAGGTATTGCCAATAATCTTGAA
CGTAGTGTAATGCAAAATATTGAACAGGCATTAAGCTCACCGCAGTCGATTGCAGAAAAC
GTATTAAAGCTTCCCAGTAAAATACTCGAGCTTGATAAAAAACTTGGCGACGACAATATT
GAAACCCAAGAGTTAATACTACTTATTGAGCAAGACCCTTTGTTGTGTGTTGAAGTGCTC
AAGTTATGTAACTCGCCCGCTTTTAAACGAGCGGATACCGATGTTAGCAGTATTCAACAA
GCGTTAGTACAAGTGGGCAGGCAACAATTAAGGCAATTTATTACCGCGTGTTTAGCCCGT
GAGTTAATCGATATAAAGCCAATTTACTTTAGACGCTTTGGGGCTGAAATCTGGCGTCAT
TCTATGCAAGTGGCTTTTTTGGCTAGCGAGTTAGCGAGCAACGAGCAACGAGACAGTGCT
TTTCTATTAGGCTTACTGCATGATGTGGGTAAAATTGCGATTTTTAAAATGCTGGTGGAC
GCTTTTTATCAAGCTGAACCAGGAGAACAACCCAATTCAATACTTTTTAAGCAGGTGATG
ACCACAAAATCGTTAACCTTAAGTGCATTGTTAGCGCGCAGTTGGCAACTCTCACAACAC
TTTGAAACACCGTTAACGTTATTGGCCAATACTAATATTAAACCAACTGATGGCATGACA
CTGCTTATTTGGAAAGCCAATATTATTAGTGAATGTTCAATGCTGTTACAAGTCGACAAA
TTGCCTCAACCAGCACTAGCGGTATTGTTAGCTCAGGTAGAGTGTAGTGAAGAAGATTTT
ATAGAATTACATCAAAAGCTTATCCAGTTTTAA

Protein sequence predicted based on nucleotide sequence

MVIGFFKKLFNVRDRNEQAKFTGITTAEKRQQTKPQTPVPAIEVTEHQAVESQLDLSALF
YSLLFATPSTTTTGIANNLERSVMQNIEQALSSPQSIAENVLKLPSKILELDKKLGDDNI
ETQELILLIEQDPLLCVEVLKLCNSPAFKRADTDVSSIQQALVQVGRQQLRQFITACLAR
ELIDIKPIYFRRFGAEIWRHSMQVAFLASELASNEQRDSAFLLGLLHDVGKIAIFKMLVD
AFYQAEPGEQPNSILFKQVMTTKSLTLSALLARSWQLSQHFETPLTLLANTNIKPTDGMT
LLIWKANIISECSMLLQVDKLPQPALAVLLAQVECSEEDFIELHQKLIQF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 394510
Recommended expression in:
Not Applicable