Mfla_0431 (M. flagellatus KT )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
48950
Keyword:
SP or TM removed
Species:
Methylobacillus flagellatus KT
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
MfCD00333139
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1530nts
Open reading frame: 1 to 1530

GGTACCATCAGCCATTCCGTCGAGCCCGGGGACAATCTTTACAACCTTGCCGCCCGCTAC
ATGGGCAATCCCCATGCCTGGCCGGATATCCAGAAACTCAACCATATTTCCAACCCACAC
CGCCTGAAGCCAGGTACCAGCCTGCTCATCCCCAAGGTCAGCAGCACTGTGACTGCCAGC
TTTGTCCATGGTGAGGCATGGCTGGTCGATGATGCAGGCAACACGATCAAACGCATCGCG
CACGGCGACCAGCTGAACGAAGGCGACATCGTCCGTACTGGCCAGCACAGCTATCTTTCG
CTGGAGTTTGCCGACCACAGTGTCGCCCGCATCCTGTCCAATACTCACGTGCGCCTGGAC
CGCATCAAGGAAAGCGGTCTCGTGCCGCAGGGCCAAAGGGTCATCTACCTTGAGCAGGGG
AACATGGACGTTTCCGTGACGCCCGCGCCCAAGTGCAATTCTCAGCATTTCAAGGTCATC
ACGCCGCAGGCCGTGGCAGCAGTGCGCGGCACGCGTTTCAGTGTGGGGGTTGGCAGTGTC
TCCGCCACCAGCGTGACGCAGGGCAAGGTGGAAATGCAGCCGCCCCAGCCAGGCAAGTCC
AGGAGCGGCAAGATGCTGCAAGCCGGGCAAGGTATTGCAGCCTCGGGCAACGGGCTCGGG
GAAGTGCAGCCATTGCTGGCAGCGCCGGACTTGAGCCAGTTGCCGTCAATGCTTCCTGAG
CCCGAATTATTGCAGTTCGACTGGCCGGCCGTGAATGGCGGGAGTGAATACCAGTACCGG
ATTGCCCAGGACGAAGGCATGGAGCAGGTGCTATGGAACGCGGCATCGTATTCCGCCAGC
GCCACGCTGCCATCCCTGCAGGATGGTGAGTACCTGTTGGCAGTACGCGCGCTGGACAGC
TATGGTATTCCGGGATACGAAGCCCAGCACCGTTTCATCATTCATAGCCATCCTTCCAGT
CCTTGGCTGCTGTCCCCTCAGCAAGGTCAACCCATTGACGGCAACGGCATCCTGCGCTGC
ACGCCGGTGGAAGGAGCACAAGGTTATCACCTGCAAATTGCCACTGACGACGAGTTCCGG
GAATTGGTGGTGGATAGTGGTCCCCTGGACCGCTGCCAATACCAGCTCGCATCGCTCGCG
GAGGGTGACTATTACTGGCGCGTCGCTTCTGTCAATGCCGAGGAAGAAGGCCGCCTGGGC
CCATACAGCCAGCCAGCCTCCCTGACCGTCAGCCAGGATGCGCATACGCTGCCTATCATC
GCAGGAGAGGCCTACTGGACAAGTACGCTGCAGGATGCCCGCTATATCGCTCAGATCAGC
CGCTCGCCCCGCTTCGACACATTGGTCGCCGAACAGGAACTGGACAGTCCACGCATCAAT
ATCCAGTCGCTGCCCGCCGGGCGTTATTACGTCAGGCTTGCCACCAGACTGGAAAACGAT
ACCGGGCCATTCTCGGATGCCCGCATCATTGAAGTAGAGCATGAGGACAATGGCATGGAT
CGCACCTGGTTTGATAAACCCAGGTCCTAA

Protein sequence predicted based on nucleotide sequence

GTISHSVEPGDNLYNLAARYMGNPHAWPDIQKLNHISNPHRLKPGTSLLIPKVSSTVTAS
FVHGEAWLVDDAGNTIKRIAHGDQLNEGDIVRTGQHSYLSLEFADHSVARILSNTHVRLD
RIKESGLVPQGQRVIYLEQGNMDVSVTPAPKCNSQHFKVITPQAVAAVRGTRFSVGVGSV
SATSVTQGKVEMQPPQPGKSRSGKMLQAGQGIAASGNGLGEVQPLLAAPDLSQLPSMLPE
PELLQFDWPAVNGGSEYQYRIAQDEGMEQVLWNAASYSASATLPSLQDGEYLLAVRALDS
YGIPGYEAQHRFIIHSHPSSPWLLSPQQGQPIDGNGILRCTPVEGAQGYHLQIATDDEFR
ELVVDSGPLDRCQYQLASLAEGDYYWRVASVNAEEEGRLGPYSQPASLTVSQDAHTLPII
AGEAYWTSTLQDARYIAQISRSPRFDTLVAEQELDSPRINIQSLPAGRYYVRLATRLEND
TGPFSDARIIEVEHEDNGMDRTWFDKPRS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 396310
Recommended expression in:
Not Applicable