UBP6 (Arabidopsis thaliana Columbia) in pVP13

Explanation of Terms

Gene:
Gene Symbol:  UBP6
Gene Name:  ubiquitin carboxyl-terminal hydrolase 6
Original Clone ID:
5786.1.28_GO.1505
Keyword:
full-length cds (mutant)
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP13
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion protein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
AtCD00334312
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1467nts
Open reading frame: 1 to 1467

GAAAACCTGTACTTCCAGTCCCCTACAGTAAGCGTGAAGTGGCAAAAGAAAGTGTTGGAT
GGTATCGAGATTGACGTTAGTCTGCCTCCTTATGTTTTCAAGGCTCAGTTGTATGATTTG
ACTGGAGTTCCACCAGAACGCCAAAAGATCATGGTCAAAGGTGGTCTCTTAAAGGATGAT
GGAGATTGGGCTGCTATTGGTGTGAAAGATGGTCAAAAATTGATGATGATGGGAACTGCT
GATGAGATAGTGAAAGCTCCTGAGAAGGCCATTGTTTTTGCAGAGGATCTACCTGAAGAA
GCGCTAGCCACTAATCTGGGTTACAGTGCTGGCCTTGTCAATCTTGGCAACACGTGTTAC
ATGAACTCCACGGTGCAGTGCTTAAAATCTGTCCCAGAGTTGAAATCTGCATTATCCAAT
TACTCACTTGCTGCCCGAAGCAATGATGTTGACCAGACTTCTCACATGCTCACAGTTGCC
ACACGTGAGTTATTTGGTGAGCTTGATAGAAGTGTCAATGCTGTTTCGCCTTCACAGTTC
TGGATGGTATTACGAAAAAAGTATCCTCAGTTTAGTCAGTTGCAGAATGGAATGCACATG
CAGCAGGATGCTGAAGAATGTTGGACACAACTGTTATACACCCTTTCTCAGTCCCTAAAA
GCACCAACTTCCAGCGAAGGTGCTGATGCTGTGAAAGCTCTATTTGGTGTCAATCTCCAG
AGCAGGTTGCATTGTCAAGAAAGTGGCGAAGAAAGCTCAGAGACAGAATCTGTATATTCT
CTAAAATGTCATATATCACATGAAGTGAACCACTTGCATGAAGGATTAAAACATGGACTG
AAAGGGGAACTTGAAAAAACATCTCCTGCTCTTGGCCGTACTGCACTCTACGTCAAGGAG
TCACTTATAGATTCCTTGCCAAGGTACTTGACTGTTCAGTTCGTGCGGTTTTTCTGGAAA
AGGGAGAGTAATCAGAAAGCAAAGATCCTCAGGAAAGTGGATTACCCGCTGGTGTTGGAT
ATATTTGACCTTTGCTCTGAGGATCTTCGGAAGAAACTGGAAGCTCCTCGCCAGAAACTT
AGAGAGGAGGAAGGTAAAAAGCTTGGTCTTCAAACTAGTGCTAAGAGTGGCTCAAAGGAC
AGTGATGTGAAAATGACTGATGCAGAGGCGTCTGCAAATGGAAGTGGAGAATCATCCACA
GTAAACCCACAGGAAGGTACTTCTTCTGAGAAGGAAACTCACATGACTGGAATCTATGAC
TTGGTCGCTGTGTTGACCCACAAGGGCAGGAGTGCAGACTCTGGTCATTATGTTGCATGG
GTCAAGCAAGAGAGTGGCAAATGGATTCAATATGATGATGACAACCCGAGCATGCAACGC
GAAGAAGACATCACAAAGCTATCTGGAGGAGGTGATTGGCATATGGCGTACATCACAATG
TACAAAGCCCGCTTCGTCTCCATGTAA

Protein sequence predicted based on nucleotide sequence

ENLYFQSPTVSVKWQKKVLDGIEIDVSLPPYVFKAQLYDLTGVPPERQKIMVKGGLLKDD
GDWAAIGVKDGQKLMMMGTADEIVKAPEKAIVFAEDLPEEALATNLGYSAGLVNLGNTCY
MNSTVQCLKSVPELKSALSNYSLAARSNDVDQTSHMLTVATRELFGELDRSVNAVSPSQF
WMVLRKKYPQFSQLQNGMHMQQDAEECWTQLLYTLSQSLKAPTSSEGADAVKALFGVNLQ
SRLHCQESGEESSETESVYSLKCHISHEVNHLHEGLKHGLKGELEKTSPALGRTALYVKE
SLIDSLPRYLTVQFVRFFWKRESNQKAKILRKVDYPLVLDIFDLCSEDLRKKLEAPRQKL
REEEGKKLGLQTSAKSGSKDSDVKMTDAEASANGSGESSTVNPQEGTSSEKETHMTGIYD
LVAVLTHKGRSADSGHYVAWVKQESGKWIQYDDDNPSMQREEDITKLSGGGDWHMAYITM
YKARFVSM*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.1505
Recommended expression in:
Not Applicable