cafA (P. aeruginosa )
In pSB3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  cafA
Gene Name:  cytoplasmic axial filament protein
Original Clone ID:
2880e1TCt3b1
Keyword:
full-length cds
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pSB3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
PaCD00336168
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1485nts
Open reading frame: 1 to 1485

AGCGAAGAGATCCTGATCAATATCACGCCGATGGAGTCGCGCGTGGCGGTGGTGGAAAAC
GGCGTCCTGCAGGAAGTGCATGTCGAACGCACCCAGCGGCGCGGCATCGTCGGCAATATC
TACAAGGGGCGGGTGGTACGGGTACTGCCGGGGATGCAGGCGGCGTTCGTCGACATCGGC
CTGGAACGTGCGGCGTTCATCCATGCCGCCGAGATATCCAATCGCGAAGGCAGCGCCGTG
GAAAGCATCAGTGCCCTGGTCCATGAGGGGCAGGCGCTGGTGGTGCAGGTGACCAAGGAT
CCGATCGGCACCAAGGGCGCACGGCTGACCACCCACCTGTCGATTCCTTCGCGCTATCTG
GTGTACATGCCGCGCACCAGCCATGTCGGGATATCCCTGCGGATCGAGGACGAAGTCGAG
CGCGAGCGCCTGAAGAAGGTGGTGGCCGACTGCGTGGCGGCGGAAGGCATCGAGGGACAG
GGCGGCTTCATCCTGCGCACCGCCGCGGAAGGTGCGGGCGAGGACGAGATTCTCGCCGAC
ATCCGCTACCTTCGCCGGCTATGGGACCAGATCGCCGCGCAGATCCAGACCGTCGGCGCG
CCTTCGGTGATCTACGAAGACCTGTCCCTGGCGATCCGTACCCTGCGCGACCTGGTGAAC
CCGCGGATCGAGAAGATCCGCATCGACTCGCGAGAGAACTTCCAGAAGATCACTTCCTTC
GTCGAAGAATTGATGCCGGAGATTTCCGATCGCCTCGAACACTACCCGGGCGAGCGACCG
ATCTTCGATCTCTACGGCGTCGAGGACGAAATCCAGAAAGCCCTGGAGCGCAAGGTACTG
CTGAAGTCCGGCGGCTACCTGATCGTCGATCCGACCGAGGCGATGACCACCATCGACGTG
AACACCGGCGCCTTCGTCGGCCATCGCAACCTCGAGGAAACCATCTTCAAGACCAACCTC
GAGGCGGCCACCGCGATCGCCCGCCAGCTGCGCCTGCGCAACCTGGGCGGGATCATCATC
ATCGACTTCATCGACATGGAGGACGAGGAGCATCGCCGGCAGGTCCTGCGCACCCTGGAG
AAGCAACTGGAGCGCGACCACGCGAAGACCAACATCATCGGCATCACCGAGTTGGGCCTG
GTGCAGATGACCCGCAAGCGCACCCGCGAAAGCCTGGTGCAGATCCTCTGCGAGCCGTGC
CCCTGCTGCCAGGGACGCGGCATGCTGAAGACTGCGGAAACCACCTGCTACGAGATTTTC
CGCGAGATTCTCCGCGAGGCGCGTGCCTACCAGGCCGACTCCTACCTGGTGCTGGCCAAC
CAGAAGGTGGTGGACCGGCTGCTCGACGAGGAGTCGGGTAACGTCGCCGATCTCGAGGCG
TTCATCGGCCGCACCATCAAGTTCCAGGTCGAGGCGATGTACTCGCAGGAGCAATATGAC
GTGGTCCTGCTCGAAGGGGGATCCCACCACCACCACCACCACTGA

Protein sequence predicted based on nucleotide sequence

SEEILINITPMESRVAVVENGVLQEVHVERTQRRGIVGNIYKGRVVRVLPGMQAAFVDIG
LERAAFIHAAEISNREGSAVESISALVHEGQALVVQVTKDPIGTKGARLTTHLSIPSRYL
VYMPRTSHVGISLRIEDEVERERLKKVVADCVAAEGIEGQGGFILRTAAEGAGEDEILAD
IRYLRRLWDQIAAQIQTVGAPSVIYEDLSLAIRTLRDLVNPRIEKIRIDSRENFQKITSF
VEELMPEISDRLEHYPGERPIFDLYGVEDEIQKALERKVLLKSGGYLIVDPTEAMTTIDV
NTGAFVGHRNLEETIFKTNLEAATAIARQLRLRNLGGIIIIDFIDMEDEEHRRQVLRTLE
KQLERDHAKTNIIGITELGLVQMTRKRTRESLVQILCEPCPCCQGRGMLKTAETTCYEIF
REILREARAYQADSYLVLANQKVVDRLLDEESGNVADLEAFIGRTIKFQVEAMYSQEQYD
VVLLEGGSHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t381c,S127S;a441g,V147V;
t684c,I228I;t762c,H254H
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-2880e
Recommended expression in:
Not Applicable