LMOf2365_0523 (L. monocytogenes serotype 4b str. F2365 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  LMOf2365_0523
Gene Name:  CocE/NonD family hydrolase
Original Clone ID:
13523a2BCt9p1
Keyword:
full-length cds
Species:
Listeria monocytogenes serotype 4b str. F2365
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
LmCD00336398
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1635nts
Open reading frame: 1 to 1635

GATTGTTCCATTAAAATGTTAAAAGATGTACCAGTTAAGATGCGTGACGGTATAACCATT
TATACCGATATTTATTTACCATTAACAGAAGAAAAAGTTCCTACTCTTATCGCCTGGAGC
CCTTATGGAAAAAGTGCTGGAACAGCTCCTCGATACAAAAATCTATTTAATATGCTAGGA
ATGGGGAATGCCTGGAATTCTGGCCTTACTAAATTTGAAGCACCTGATCCAGCATACTGG
TGCGCACATGGATACGCTGTCTGTAATCCTGACATGCGTGGCATTGCTCATAGTGAGGGT
AATACTACAATGATTGGCTCTCAAGAAGCTGAAGATGGCTATGATTTAATTGAATGGTTA
GCTAAGCAATCTTGGAGTAATGGAAAAACAGCTCTAACAGGAACATCTTATTTAGCCTTT
TCCCAGTGGTATATTGCAGCTGAGCAGCCCCCTCACCTAACATGTATCAATCCTACTGAA
GGATTAGCAGATGGTTATCGTGACTTAGCTTTTATTGGAGGCATTCCAGATCCTAATTTT
ATTGAGCGTTTGCAAATCAACCATGTCAGTGCTAAAAATTCGCAAAGAGAAGATTTAACA
AAGGAAATGGAAGCATATCCTTTAGCTGATAGTGCTATTTGGAAAGACAAAGTTGCAGAT
CCAAGTAAAATTACAATACCAGCTTTTGTTATTGCAAGTTATTCTAATACGCTTCACACA
ATGGGTACATTTCGTTCTTGGCGTTCACTTGGTTCTAAAGAAAAATGGCTTCGTATTCAT
GATCGCCAAGAATGGCCATATTACTATGACGAAAATAACGTAGAAGAATTGCGACGCTTT
TTTGATTATTATTTACTTGGCAAGGAAAATAATTGGACAGATACACCAACTGTGCGCTAT
GCATTAATTGACTTTCAAGGTGGCAATCAAACTGACTTACCATCCGAAGTTTTTCCACCT
ACCAATAGTGAAAACAAACGTTATTATATGAATGGCCTATTACGTACTTTACAAGAAACA
CCTACTTCAGATGATTTTAAAGTATCCTATACTACTGAAAACTTGCCAGGGAGAGCATCG
TTCCAAATGACGTTTGACAAAGAAACGCATTTTGTTGGTTATCCAAAAGCAAAATTATTT
ATGGAAGTAGATGGTTACGATGACTTGGATGTTTTTGTTTGGCTTCAAAAGCTAGATAAA
TTCGGCAACGTACTAAGTGAATTTGTCGTACCAAATCACGGGGCCGCACTTCAGGATTTC
ACACAAGAAGGGGCATCTGCTTTACGCTATAAAGGCGCTTGGGGAAGACTTCGCGCATCC
ATGCGACAACTAGATTCTCAAGTTTCAACAGATGAAATTCCTGCTTATAGCTTTGACAAA
GTAGAAAAACTCTCTTCCGGAGAAATTGTAGAACTAGACATTATTTTGAGTCCCATTGGT
CTAACTTATAAAGCAGGTGAAACACTCAGAGTAGTCGTGAGTAGCAAAGATGAACTTGGC
TCAGTAATGCCAGGAACGCCCGGATGCACACCTAATAATAAAGGTATGCATATCCTTCAT
ACGAGTAAAGAGCACCCATCCTATATTCAGCTACCAATAATCAACAAAGAAGGGCATCAT
CACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

DCSIKMLKDVPVKMRDGITIYTDIYLPLTEEKVPTLIAWSPYGKSAGTAPRYKNLFNMLG
MGNAWNSGLTKFEAPDPAYWCAHGYAVCNPDMRGIAHSEGNTTMIGSQEAEDGYDLIEWL
AKQSWSNGKTALTGTSYLAFSQWYIAAEQPPHLTCINPTEGLADGYRDLAFIGGIPDPNF
IERLQINHVSAKNSQREDLTKEMEAYPLADSAIWKDKVADPSKITIPAFVIASYSNTLHT
MGTFRSWRSLGSKEKWLRIHDRQEWPYYYDENNVEELRRFFDYYLLGKENNWTDTPTVRY
ALIDFQGGNQTDLPSEVFPPTNSENKRYYMNGLLRTLQETPTSDDFKVSYTTENLPGRAS
FQMTFDKETHFVGYPKAKLFMEVDGYDDLDVFVWLQKLDKFGNVLSEFVVPNHGAALQDF
TQEGASALRYKGAWGRLRASMRQLDSQVSTDEIPAYSFDKVEKLSSGEIVELDIILSPIG
LTYKAGETLRVVVSSKDELGSVMPGTPGCTPNNKGMHILHTSKEHPSYIQLPIINKEGHH
HHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a82t,I28L;t450c,P150P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-13523a
Recommended expression in:
Not Applicable