BF1045 (B. fragilis )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BF1045
Gene Name:  transglutaminase
Original Clone ID:
12019a5BCt4p1
Keyword:
full-length cds
Species:
Bacteroides fragilis
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BfCD00336474
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1047nts
Open reading frame: 1 to 1047

GGAGAGTTGTTTGCAGTGTTTAATGAAAAGCTCACCATTCCCGAACAAGAGGCTTTAATG
TTCCTCTATGCCTACATGCCTACAGGTGACGTAACGGATTATACAGGCGACTACTATCTG
GAAAATGTCAGACTCTCCGATCAGGCACGTCGGGAAATGCCTTGGGGAAAAGAAATCCCC
GATGACGTATTCCGTCATTTTGTGCTCCCCATTCGGGTAAACAATGAAAACCTGGATGAC
TCACGCCGGGTGTTTTACAATGAACTGAAAGACCGTGTGAAGAATCTGTCATTACACGAC
GCTGTACTCGAGGTAAACCACTGGTGCCACGAAAAGGTAATCTACACCCCAAGTGATGCC
CGTACGAGTGCTCCACTCGCTTCCGTAAAAACCGCTTATGGTCGCTGTGGTGAAGAATCC
ACCTTCACCGTAGCAGCTCTTCGCTCGGTAGGTATTCCGGCACGACAGGTATATACTCCG
CGTTGGGCACATACCGATGACAACCATGCATGGGTAGAAGCCTGGGTAGATGGCAAATGG
TATTTCTTTGGTGCCTGTGAACCGGAACCGGTTTTGAACCTGGGTTGGTTTAATGCTCCC
GCCAGTCGTGGTATGCTGATGCATACAAAGGTATTCGGACGCTATACCGGACAAGAAGAA
ATCATGTACGAAACTCCGAATTATACAGAGATCAACGTGATTGACAATTATGCTCCGACT
GCCAAAGGTTCCGTACTCGTGACAGATGCCGAAGGTCAGCCGGTAGCCGATGCTACCGTA
GAGTTCAAGGTCTATAACTACGCTGAATTTTATACGGTGGCCACCAAACATACAGACCGG
AGCGGGCATGCATCATTGACTGCCGGCAAGGGTGATATGTTGGTATGGGCCTCCAAAGAC
GGACGGTTCGGTTATTCTAAACTATCATTCGGCAAAGACAATGAACTGAAGATCACACTG
GACAAAAACGCCAGTGAAACCTATTCGCTTCCACTGGATATCGTTCCTCCTGCCGAAGGC
GAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

GELFAVFNEKLTIPEQEALMFLYAYMPTGDVTDYTGDYYLENVRLSDQARREMPWGKEIP
DDVFRHFVLPIRVNNENLDDSRRVFYNELKDRVKNLSLHDAVLEVNHWCHEKVIYTPSDA
RTSAPLASVKTAYGRCGEESTFTVAALRSVGIPARQVYTPRWAHTDDNHAWVEAWVDGKW
YFFGACEPEPVLNLGWFNAPASRGMLMHTKVFGRYTGQEEIMYETPNYTEINVIDNYAPT
AKGSVLVTDAEGQPVADATVEFKVYNYAEFYTVATKHTDRSGHASLTAGKGDMLVWASKD
GRFGYSKLSFGKDNELKITLDKNASETYSLPLDIVPPAEGEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-12019a
Recommended expression in:
Not Applicable