c4920 (E. coli )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  c4920
Gene Name:  starvation sensing protein rspA
Original Clone ID:
9262c1BCt5p1
Keyword:
full-length cds
Species:
Escherichia coli
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
EcCD00336723
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1236nts
Open reading frame: 1 to 1236

AAAATTATCGCGGTGGACGTGTTTGTCACCTGCCCGGGCCGTAATTTCGTAACGCTGAAG
ATAACTACAGAAAGCGGTTTATGTGGGTTGGGAGACGCCACCCTTAACGGCCGCGAGCTC
TCTGTGGCAAGTTATCTGAAAGATCATCTGTGCCCACAGTTAATTGGTCGGGACGCTTCA
AGAATCGAAGATATTTGGCAATTTTTCTACAAAGGGGCGTATTGGCGACGTGGACCTGTT
ACGATGTCCGCCATCTCGGCAATAGACATGGCTTTGTGGGATATTAAGGCGAAAGCCGCA
AATATGCCCCTTTACCAGCTGCTCGGCGGTGCTAGCCGCGAGGGCGTCATGGTATATTGT
CACACTACAGGTCGTACCATCGATGAAGTGCTGGAGGACTACGCGAAACATCAACAGATG
GGGTTTAAGGCCATTCGCGTTCAGTGCGGAGTCCCGGGCATGCAAACGACCTATGGGTTA
GCAAAAGGCAAAGGACTTGCTTACGAGCCAGCGACTAAGGGGTTGTGGCCTGAAGAGCAG
CTTTGGTCTAGTGAAAAATATCTGGATTTCACACCGAAACTCTTTGAAGCCGTGCGGAAC
AAGTTCGGATTTAATGAGCACTTACTGCATGACATGCACCATCGTTTGACGCCCATAGAA
GCAGCGCGCTTCGGCAGATCAATCGAGGATTATCGACTTTTTTGGATGGAGGACCCGACC
CCAGCTGAAAACCAAGAGTGTTTTCGTCTGATTCGCCAGCACACTGTAACACCTATCGCC
GTTGGTGAAGTGTTCAATTCCATTTGGGATTGCAAACAGTTAATAGAGGAACAACTGATC
GATTACATTCGGACCACGATAACCCATGCAGGGGGCATCACTGGTATGCGTCGCATTGCG
GACTTTGCTTCGCTCTATCAGGTCCGTACAGGAAGCCACGGCCCGTCTGATCTGAGTCCC
GTTTGTCATGCCGCAGCGTTGCACTTCGACCTTTGGGTACCGAACTTTGGTGTGCAAGAA
TACATGGGGTATTCAGAGCAGATGCTGGAAGTATTCCCACATTCCTGGCGCTTTGATGAA
GGCTACATGCATCCTGGTGATGAGCCGGGATTAGGCATTTCGTTCGACGAAAAACTGGCT
GCCAAGTATCCATACGATCCGGCATATCTCCCCGTTGCGAGATTGGAGGACGGTACTTTA
TGGAATTGGGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

KIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDAS
RIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYC
HTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQ
LWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGRSIEDYRLFWMEDPT
PAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRIA
DFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFDE
GYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGTLWNWEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a396g,E132E;t477g,G159G;
t495a,G165G;g498t,L166L;
a711g,E237E;c1053a,V351V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9262c
Recommended expression in:
Not Applicable