PSMD13 (Homo sapiens) in pVP16

Explanation of Terms

Gene:
Gene Symbol:  PSMD13
Gene Name:  proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Original Clone ID:
5881.1.80_GO.36487
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pVP16
Format: 
FUSION
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion protein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
HsCD00336732
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1149nts
Open reading frame: 1 to 1149

GAAAACCTGTACTTCCAGTCCAAGGACGTACCGGGCTTCCTACAGCAGAGCCAGAGCTCC
GGGCCCGGGCAGCCCGCTGTGTGGCACCGTCTGGAGGAGCTCTACACGAAGAAGTTGTGG
CATCAGCTGACACTTCAGGTGCTTGATTTTGTGCAGGATCCGTGCTTTGCCCAAGGAGAT
GGTCTCATTAAGCTTTATGAAAACTTTATCAGTGAATTTGAACACAGGGTGAATCCTCTG
TCCCTCGTGGAAATCATTCTTCACGTAGTTAGACAGATGACTGATCCTAATGTGGCTCTT
ACTTTTCTGGAAAAGACTCGTGAGAAGGTGAAAAGTAGTGATGAGGCAGTGATCCTGTGT
AAAACAGCAATTGGAGCTCTAAAATTAAACATCGGGGACCTACAGGTTACAAAGGAAACA
ATTGAAGATGTTGAAGAAATGCTCAACAACCTTCCTGGTGTGACATCGGTTCACAGTCGT
TTCTATGATCTCTCCAGTAAATACTATCAAACAATCGGAAACCACGCGTCCTACTACAAA
GATGCTCTGCGGTTTTTGGGCTGTGTTGACATCAAGGATCTACCAGTGTCTGAGCAGCAG
GAGAGAGCCTTCACGCTGGGGCTAGCAGGACTTCTCGGCGAGGGAGTTTTTAACTTTGGA
GAACTCCTCATGCACCCTGTGCTGGAGTCCCTGAGGAATACTGACCGGCAGTGGCTGATT
GACACCCTCTATGCCTTCAACAGTGGCAACGTAGAGCGGTTCCAGACTCTGAAGACTGCC
TGGGGCCAGCAGCCTGATTTAGCAGCTAATGAAGCCCAGCTTCTGAGGAAAATTCAGTTG
TTGTGCCTCATGGAGATGACTTTCACACGACCTGCCAATCACAGACAACTCACTTTTGAA
GAAATTGCCAAAAGTGCTAAAATCACAGTGAATGAGGTGGAGCTTCTGGTGATGAAGGCC
CTTTCGGTGGGGCTGGTGAAAGGCAGTATAGACGAGGTGGACAAACGAGTCCACATGACC
TGGGTGCAGCCCCGAGTGTTGGATTTGCAACAGATCAAGGGAATGAAGGACCGCCTGGAG
TTCTGGTGCACGGATGTGAAGAGCATGGAGATGCTGGTGGAGCACCAGGCCCATGACATC
CTCACCTAGTAG

Protein sequence predicted based on nucleotide sequence

ENLYFQSKDVPGFLQQSQSSGPGQPAVWHRLEELYTKKLWHQLTLQVLDFVQDPCFAQGD
GLIKLYENFISEFEHRVNPLSLVEIILHVVRQMTDPNVALTFLEKTREKVKSSDEAVILC
KTAIGALKLNIGDLQVTKETIEDVEEMLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYK
DALRFLGCVDIKDLPVSEQQERAFTLGLAGLLGEGVFNFGELLMHPVLESLRNTDRQWLI
DTLYAFNSGNVERFQTLKTAWGQQPDLAANEAQLLRKIQLLCLMEMTFTRPANHRQLTFE
EIAKSAKITVNEVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKGMKDRLE
FWCTDVKSMEMLVEHQAHDILT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
ins@1146,3
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.36487
Recommended expression in:
Not Applicable