AT2G18780 (Arabidopsis thaliana Columbia) in pVP13

Explanation of Terms

Gene:
Gene Symbol:  AT2G18780
Gene Name:  F-box protein
Original Clone ID:
5786.1.32_GO.8595
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP13
Format: 
FUSION
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion protein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
AtCD00336794
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1110nts
Open reading frame: 1 to 1110

GAAAACCTGTACTTCCAGTCCCTCTCGGATCTTCCAAAGGATGTGGCGGAGGAGGTGCTC
TCGAAGTTTCCAGTGACATCTCTGAGAAGAGTACGATTCACATGCAAAAAGTTGAACACT
TTATCCAAAGATATGTCTTTTACAAGGAAGCACATCGATAAAGAAGAAGCAAAGAAGAAG
CAGAGTAAGGAGTTTCATGTTGTGATGATGCTCGATTTTAGGGTTTCTTTATTCAGTCTC
AATCTACTAAACCCTAATCCCATAGAGCGTTTAGGTCAACTTGTTAGCTTAGACGGTGGT
GCACAAGTCGACATATCTAACATTTTTCACTGCGAGGGTTTCTTGTTATGCACCACTAAA
GACATATCTAGGGTTGTTGTTTGGAACCCTTGTGTTGGGCAAACAATTTGGATCAAACCC
AGAAACTCTTTCAACAAATGGGATAGGTATGCTCTGGGCTACGATGTGATGAAGAATCAT
AAAGTTTTGAGATTTGTGGATGACGGTGAGTATGGAAGGAGACACTTACTTTGTGAGTTT
GAAATCTACAGTTTTGAGTCTGATTCATGGGAGGTTCTTGATGTGAATCCGGATTGGGAG
ATAGATTTTGTTCACCGTGGTTTGTCTATCGATGGAAACAGTTACTGGTTTGCACGAGAC
AAGGTTGTTCCATATGGAGGACAAGATCCTTCTTATTTCTTGCTGTGTTTTGATTTCACA
ACGGAGAGATTTAGACCGCCTCTGCCTCTGCCATTTCAGCCTTACTTTGGTGATACTGTG
GCTCTATCTAGCGTTGGGGATAACCAGATTGCGGTCTTGTGTCAGCGGTCTTCTTCGCCG
TATACCTTGAAGATATGGATTAGCAGCAAGGTTGAGCCCAATGCAGTGTCGTGGAACAAG
TTGTTCTTAAAGGTTGACATGAAACCTCTCACTGGTAATTCCTTCTCTTATTCGGGTGGG
AGTTTCTTTGTTGACGAGGAGAAGAAGATGGCTGTGGTTCTTGATAAAAACATAGGTGGT
GGATTTGACCCGACTCGCAACATAGCTTACATCATTGGAAAGGAGGGCTACTTCAAGAAA
GTTGATCTTGGCGAATCTAGGCACCTCTAGTAG

Protein sequence predicted based on nucleotide sequence

ENLYFQSLSDLPKDVAEEVLSKFPVTSLRRVRFTCKKLNTLSKDMSFTRKHIDKEEAKKK
QSKEFHVVMMLDFRVSLFSLNLLNPNPIERLGQLVSLDGGAQVDISNIFHCEGFLLCTTK
DISRVVVWNPCVGQTIWIKPRNSFNKWDRYALGYDVMKNHKVLRFVDDGEYGRRHLLCEF
EIYSFESDSWEVLDVNPDWEIDFVHRGLSIDGNSYWFARDKVVPYGGQDPSYFLLCFDFT
TERFRPPLPLPFQPYFGDTVALSSVGDNQIAVLCQRSSSPYTLKIWISSKVEPNAVSWNK
LFLKVDMKPLTGNSFSYSGGSFFVDEEKKMAVVLDKNIGGGFDPTRNIAYIIGKEGYFKK
VDLGESRHL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
ins@1107,3
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in GO.8595
Recommended expression in:
Not Applicable