UBP14 (Arabidopsis thaliana Columbia) in pVP13

Explanation of Terms

Gene:
Gene Symbol:  UBP14
Gene Name:  ubiquitin carboxyl-terminal hydrolase 14
Original Clone ID:
5444.1.16_GO.12193
Keyword:
full-length cds (mutant)
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP13
Format: 
FUSION
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
His8-MBP fusion protein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
AtCD00336821
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1383nts
Open reading frame: 1 to 1383

GAAAACCTGTACTTCCAGTCCGCTTTTGAGATGGCTCCTGCTGATCCAACTTTGGACCTC
AATATGCAATTAACAAAGCTTGGACACGGCTTACTATCTGGGAAGTACTCAATGCCTGCA
ACTCAGAAGGATGCTACAACTGGAGATCCTAGACAAGAAGGAATACCTCCTCGCATGTTC
AAAAACGTAATTGCCGCGAGCCACGCAGAATTCTCTTCTATGAGACAGCAGGATGCTCTC
GACTTTTTCCTCCATCTGGTAGGCAAGGTTGAGCGTGCTAGCAACACGACACCAGATTTA
GACCCCTCGAGGAGCTTCAAGTTTGGAATAGAAGAGAAGATCCTTTGTCCGTCTGGAAAG
GTTGGATATAATAAGAGGGAAGACTGTATTCTTTCTTTGAACATTCCGCTACATGAGGCA
ACTAATAAAGATGAATTAGAAGCCTTTCACAAGCAAAAAGCAGGAAAAGGATTGGAAGAG
AATGATATGAGGTCAAGTGATGAAATAGTACGCCCACGAGTTCCTTTAGAAGCCTGTCTA
GCAAATTTTGCATCATCAGAGCCGATTGAAGACTACTATAGCTCTGCTTTGAAGGGAATG
ACAACAGCTATCAAGACAACTGGTTTGACATCTTTCCCAGATTATTTGGTCTTGCACATG
CGGAAATTTGTTATGGAGGAAGGCTGGGTGCCAAAGAAACTTGATGTATACATTGATGTT
CCGGATGTTATTGATATCAGCCACATGCGTAGCAAAGGACTCCAACCTGGGGAAGAACTG
TCGCCAGATGGCGTTCCAGAAGAAGTGATGGAATCAGCGCAGCCCGTGGCAAATGAGGAG
ATAGTTGCACAGCTAGTCTCAATGGGATTTAGCCAGCTTCACTGCCAGAAAGCTGCCATA
AATACTTCCAATGCTGGGGTTGAAGAGGCAATGAACTGGTTACTTTCTCACATGGATGAT
CCAGACATCGATGCACCAATCTCCCACCAGACATCTGACATTGATCAATCAAGCGTTGAT
ACCTTACTCTCCTTTGGTTTTGCTGAAGATGTTGCTCGGAAGGCACTAAAAGCCTCGGGA
GGAGACATTGAGAAAGCAACAGACTGGGTATTCAACAACCCTAATGCATCTGTTTCAGAC
ATGGATGTATCCTCTAGCAATTCAGCGCAGACTCCGGCTCAAAGTGGATTACCAGATGGA
GGAGGGAAATACAAGCTGTTTGGAATAGTAAGTCACATGGGAACATCAGTGCACTGTGGT
CATTACGTGGCTCACATATTGAAAGAAGGCCGCTGGGTAATTTTCAATGACGACAAAGTT
GGTATCTCGACTGATCCTCCTAAAGACATGGGTTATGTCTACTTCTTTCAGCGGCTTGAT
TGATAG

Protein sequence predicted based on nucleotide sequence

ENLYFQSAFEMAPADPTLDLNMQLTKLGHGLLSGKYSMPATQKDATTGDPRQEGIPPRMF
KNVIAASHAEFSSMRQQDALDFFLHLVGKVERASNTTPDLDPSRSFKFGIEEKILCPSGK
VGYNKREDCILSLNIPLHEATNKDELEAFHKQKAGKGLEENDMRSSDEIVRPRVPLEACL
ANFASSEPIEDYYSSALKGMTTAIKTTGLTSFPDYLVLHMRKFVMEEGWVPKKLDVYIDV
PDVIDISHMRSKGLQPGEELSPDGVPEEVMESAQPVANEEIVAQLVSMGFSQLHCQKAAI
NTSNAGVEEAMNWLLSHMDDPDIDAPISHQTSDIDQSSVDTLLSFGFAEDVARKALKASG
GDIEKATDWVFNNPNASVSDMDVSSSNSAQTPAQSGLPDGGGKYKLFGIVSHMGTSVHCG
HYVAHILKEGRWVIFNDDKVGISTDPPKDMGYVYFFQRLD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
del@1384,3
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.12193
Recommended expression in:
Not Applicable