DNASU Plasmid | Detailed Plasmid Information

Explanation of Terms

Gene:

Gene Symbol: ANKRD2

Gene Name: ankyrin repeat domain 2 (stretch responsive muscle)

Original Clone ID:

HR3134-21.6

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET21_NESG

Format:

FUSION

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI
Northeast Structural Genomics Consortium

HsCD00338622

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 999nts

Open reading frame: 1 to 999

Reference Sequence Alignment

ATGGACGGCACCATGGAGGACTCCGAGGCGGTGCAGAGGGCCACAGCGCTCATCGAGCAG
CGGCTGGCACAGGAGGAGGAGAATGAGAAACTCCGAGGAGACGCACGCCAGAAGCTGCCC
ATGGACTTGCTGGTGCTGGAGGATGAGAAGCACCACGGGGCTCAGAGTGCAGCCCTGCAG
AAGGTGAAGGGCCAAGAGCGCGTGCGCAAGACGTCCCTGGACCTGCGGCGGGAGATCATC
GATGTGGGCGGGATCCAGAACCTCATCGAGCTGCGGAAGAAACGCAAGCAGAAGAAGCGG
GACGCTCTGGCCGCCTCGCATGAGCCGCCCCCAGAGCCCGAGGAGATCACTGGCCCTGTG
GATGAGGAGACCTTCCTGAAAGCTGCGGTGGAGGGGAAAATGAAGGTCATTGAGAAGTTC
CTGGCTGACGGGGGGTCAGCCGACACGTGCGACCAGTTCCGTCGGACAGCACTGCACCGA
GCTTCCCTGGAAGGCCACATGGAAATCCTGGAGAAGCTTCTAGATAATGGGGCCACTGTG
GACTTCCAGGATCGGCTGGACTGCACAGCCATGCATTGGGCCTGCCGCGGGGGCCACTTA
GAGGTGGTGAAACTTCTGCAAAGCCATGGAGCAGACACCAATGTGAGGGATAAGCTGCTG
AGCACCCCGCTGCACGTGGCAGTCCGGACAGGGCAGGTGGAGATTGTGGAGCACTTTCTA
TCCCTGGGCCTGGAAATCAATGCCAGAGACAGGGAAGGGGATACTGCCCTGCATGACGCT
GTGAGGCTCAACCGCTACAAAATCATCAAACTGCTGCTCCTGCATGGGGCTGACATGATG
ACCAAGAACCTGGCAGGAAAGACCCCGACGGACCTGGTGCAGCTCTGGCAGGCTGATACC
CGGCACGCCCTGGAGCATCCTGAGCCGGGGGCTGAGCATAACGGGCTGGAGGGGCCTAAT
GATAGTGGGCGAGAGGCCCCTCAGCCTGTGCCAGCCCAG

Protein sequence predicted based on nucleotide sequence

MDGTMEDSEAVQRATALIEQRLAQEEENEKLRGDARQKLPMDLLVLEDEKHHGAQSAALQ
KVKGQERVRKTSLDLRREIIDVGGIQNLIELRKKRKQKKRDALAASHEPPPEPEEITGPV
DEETFLKAAVEGKMKVIEKFLADGGSADTCDQFRRTALHRASLEGHMEILEKLLDNGATV
DFQDRLDCTAMHWACRGGHLEVVKLLQSHGADTNVRDKLLSTPLHVAVRTGQVEIVEHFL
SLGLEINARDREGDTALHDAVRLNRYKIIKLLLLHGADMMTKNLAGKTPTDLVQLWQADT
RHALEHPEPGAEHNGLEGPNDSGREAPQPVPAQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a103g,T35A;a976g,T326A

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR3134

Recommended expression in:

Not Applicable

Vector Name:

pET21_NESG

Synonyms:

pET21, pET21-modified, pET21Cc, pET21_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5415

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Thomas B. Acton
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCCCCGCCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI)

157

253

bacterial origin

ori

pBR322-type bacterial origin of replication

3180

3862

gene fragment

ROP

2580

2756

primer

T7 forward

T7 forward sequencing primer

283

298

primer

T7 reverse

T7 reverse sequencing primer

promoter

T7 promoter

280

299

repressor binding site

lacO

lacO regulatory sequence

258

280

repressor protein gene

LacI

752

1768

selectable marker

AmpR

ampicillin resistance gene

3960

4619

ssDNA origin

f1 ori

f1 origin

5081

5387

tag

His

C-terminal 6xHis tag

140

157

trxn termination sequence

T7 term

T7 termination sequence

Gene

Gene Symbol :

ANKRD2

Symbol Nomenclature :

ANKRD2

SYNONYM :

ARPP

Designation :

ankyrin repeat domain-containing protein 2|ankyrin-repeat protein|hArpp|skeletal muscle ankyrin repeat protein

Full Nomenclature :

ankyrin repeat domain 2 (stretch responsive muscle)

GENEID :

26287

Locus Tag :

RP11-548K23.2

HGNC :

495

MIM :

610734

Vega :

OTTHUMG00000018860

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00338622      ------------------------------------------------------------
NM_020349.2       ATGGCAAAGGCGCCCAGCTGGGCAGGGGTGGGTGCTCTGGCCTATAAAGCCCCCGAGGCC
                                                                              

HsCD00338622      ---------------------ATGGACGGCACCATGGAGGACTCCGAGGCGGTGCAGAGG
NM_020349.2       CTGTGGCCTGCAGAGGCGGTTATGGACGGCACCATGGAGGACTCCGAGGCGGTGCAGAGG
                                       ***************************************

HsCD00338622      GCCACAGCGCTCATCGAGCAGCGGCTGGCACAGGAGGAGGAGAATGAGAAACTCCGAGGA
NM_020349.2       GCCACAGCGCTCATCGAGCAGCGGCTGGCACAGGAGGAGGAGAATGAGAAACTCCGAGGA
                  ************************************************************

HsCD00338622      GACGCACGCCAGAAGCTGCCCATGGACTTGCTGGTGCTGGAGGATGAGAAGCACCACGGG
NM_020349.2       GACGCACGCCAGAAGCTGCCCATGGACTTGCTGGTGCTGGAGGATGAGAAGCACCACGGG
                  ************************************************************

HsCD00338622      GCTCAGAGTGCAGCCCTGCAGAAGGTGAAGGGCCAAGAGCGCGTGCGCAAGACGTCCCTG
NM_020349.2       GCTCAGAGTGCAGCCCTGCAGAAGGTGAAGGGCCAAGAGCGCGTGCGCAAGACGTCCCTG
                  ************************************************************

HsCD00338622      GACCTGCGGCGGGAGATCATCGATGTGGGCGGGATCCAGAACCTCATCGAGCTGCGGAAG
NM_020349.2       GACCTGCGGCGGGAGATCATCGATGTGGGCGGGATCCAGAACCTCATCGAGCTGCGGAAG
                  ************************************************************

HsCD00338622      AAACGCAAGCAGAAGAAGCGGGACGCTCTGGCCGCCTCGCATGAGCCGCCCCCAGAGCCC
NM_020349.2       AAACGCAAGCAGAAGAAGCGGGACGCTCTGGCCGCCTCGCATGAGCCGCCCCCAGAGCCC
                  ************************************************************

HsCD00338622      GAGGAGATCACTGGCCCTGTGGATGAGGAGACCTTCCTGAAAGCTGCGGTGGAGGGGAAA
NM_020349.2       GAGGAGATCACTGGCCCTGTGGATGAGGAGACCTTCCTGAAAGCTGCGGTGGAGGGGAAA
                  ************************************************************

HsCD00338622      ATGAAGGTCATTGAGAAGTTCCTGGCTGACGGGGGGTCAGCCGACACGTGCGACCAGTTC
NM_020349.2       ATGAAGGTCATTGAGAAGTTCCTGGCTGACGGGGGGTCAGCCGACACGTGCGACCAGTTC
                  ************************************************************

HsCD00338622      CGTCGGACAGCACTGCACCGAGCTTCCCTGGAAGGCCACATGGAAATCCTGGAGAAGCTT
NM_020349.2       CGTCGGACAGCACTGCACCGAGCTTCCCTGGAAGGCCACATGGAAATCCTGGAGAAGCTT
                  ************************************************************

HsCD00338622      CTAGATAATGGGGCCACTGTGGACTTCCAGGATCGGCTGGACTGCACAGCCATGCATTGG
NM_020349.2       CTAGATAATGGGGCCACTGTGGACTTCCAGGATCGGCTGGACTGCACAGCCATGCATTGG
                  ************************************************************

HsCD00338622      GCCTGCCGCGGGGGCCACTTAGAGGTGGTGAAACTTCTGCAAAGCCATGGAGCAGACACC
NM_020349.2       GCCTGCCGCGGGGGCCACTTAGAGGTGGTGAAACTTCTGCAAAGCCATGGAGCAGACACC
                  ************************************************************

HsCD00338622      AATGTGAGGGATAAGCTGCTGAGCACCCCGCTGCACGTGGCAGTCCGGACAGGGCAGGTG
NM_020349.2       AATGTGAGGGATAAGCTGCTGAGCACCCCGCTGCACGTGGCAGTCCGGACAGGGCAGGTG
                  ************************************************************

HsCD00338622      GAGATTGTGGAGCACTTTCTATCCCTGGGCCTGGAAATCAATGCCAGAGACAGGGAAGGG
NM_020349.2       GAGATTGTGGAGCACTTTCTATCCCTGGGCCTGGAAATCAATGCCAGAGACAGGGAAGGG
                  ************************************************************

HsCD00338622      GATACTGCCCTGCATGACGCTGTGAGGCTCAACCGCTACAAAATCATCAAACTGCTGCTC
NM_020349.2       GATACTGCCCTGCATGACGCTGTGAGGCTCAACCGCTACAAAATCATCAAACTGCTGCTC
                  ************************************************************

HsCD00338622      CTGCATGGGGCTGACATGATGACCAAGAACCTGGCAGGAAAGACCCCGACGGACCTGGTG
NM_020349.2       CTGCATGGGGCTGACATGATGACCAAGAACCTGGCAGGAAAGACCCCGACGGACCTGGTG
                  ************************************************************

HsCD00338622      CAGCTCTGGCAGGCTGATACCCGGCACGCCCTGGAGCATCCTGAGCCGGGGGCTGAGCAT
NM_020349.2       CAGCTCTGGCAGGCTGATACCCGGCACGCCCTGGAGCATCCTGAGCCGGGGGCTGAGCAT
                  ************************************************************

HsCD00338622      AACGGGCTGGAGGGGCCTAATGATAGTGGGCGAGAGGCCCCTCAGCCTGTGCCAGCCCAG
NM_020349.2       AACGGGCTGGAGGGGCCTAATGATAGTGGGCGAGAGACCCCTCAGCCTGTGCCAGCCCAG
                  ************************************.***********************

HsCD00338622      ---
NM_020349.2       TGA