DSY3815 (D. hafniense )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  DSY3815
Gene Name:  hypothetical protein
Original Clone ID:
DhR85C-21.1
Keyword:
targeted domain
Species:
Desulfitobacterium hafniense
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
DhCD00338938
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 354nts
Open reading frame: 1 to 354

ATGAAAAATTTTCTGGAAACGATTGAGGATATGATCCTCATTATTAATCGGGAAGGAAGA
TTATTATATGCAAATACTGCCGTGCCTAAGAAGCTGGGCTATACTCATGAAGAATTAATG
AGCATGCACATCCTGACCATTACCAGCGCCGGTAAGATGGCTGAAGGTGAAAAGATCTTA
GCTGAGCTGTTTGCGGGCAAAAAAGAGAGTCTCCCTTTGTCCCTGGAAAAAAAGGAGGGA
ACCTCCATCCCTGCAAAAGCGCGTATCTGGCAGGGGAAGTGGCATAATGAGCCTTGTCTT
TTTGCCATCATTAAGGATTTGTCAAAAGAAGAAAGAGCTTCTTCACCCCCATTC

Protein sequence predicted based on nucleotide sequence

MKNFLETIEDMILIINREGRLLYANTAVPKKLGYTHEELMSMHILTITSAGKMAEGEKIL
AELFAGKKESLPLSLEKKEGTSIPAKARIWQGKWHNEPCLFAIIKDLSKEERASSPPF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g87t,P29P;c103a,P35T;
t129c,H43H;t177c,I59I;
a207g,E69E
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in DhR85C
Recommended expression in:
Not Applicable