VP2106 (V. parahaemolyticus )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VP2106
Gene Name:  electron transport complex protein RnfG
Original Clone ID:
VpR279-51-203-21.12
Keyword:
full length protein truncation
Species:
Vibrio parahaemolyticus
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
VpCD00339846
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 462nts
Open reading frame: 1 to 462

ATGCCTGAAACGATGCACGACAACGCATTAACTCAATCATGCACGTTGGTGACGTCTCCA
GAGCTTGGCACGATGCACGCAATGCCTACCTACATTGCCACCAAAAATGGCGAGCCAACA
GCCATCGCCATTGAGTCGATTGCACCAGATGGCTATAACGGTGAAATCAAAGTCATCACA
GGTATCGATAATCAAGGTAAGATTTTAGGTACGCGCGTACTTAGCCATCAAGAAACACCG
GGGCTAGGCGATAAAATAGATCTTCGCGTTACCAGTTGGATTCTTGGCTTCACGGGTAAG
CAAGTCACCGAAGATAATTGGAATTCTTGGAAAGTTCGTAAAGATGGTGGAGACTTTGAT
CAGTTTACTGGCGCTACCATTACGCCTCGAGCTGTCGTCAAAGCAGTCAGAAACACCGTA
AACTACGTAAATCAATCTCGTGATGAGATTCTCAGCCAGCCA

Protein sequence predicted based on nucleotide sequence

MPETMHDNALTQSCTLVTSPELGTMHAMPTYIATKNGEPTAIAIESIAPDGYNGEIKVIT
GIDNQGKILGTRVLSHQETPGLGDKIDLRVTSWILGFTGKQVTEDNWNSWKVRKDGGDFD
QFTGATITPRAVVKAVRNTVNYVNQSRDEILSQP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t45g,T15T;a54g,T18T;
g81a,A27A;a397g,I133V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in VpR279
Recommended expression in:
Not Applicable