VP2536 (V. parahaemolyticus RIMD 2210633 )
In pNYCOMPS-LIC-FH10T+ (N term)

Explanation of Terms

Gene:
Gene Symbol:  VP2536
Gene Name:  hemolysin
Original Clone ID:
GE.349.1.67_GO.8625
Keyword:
None
Species:
Vibrio parahaemolyticus RIMD 2210633
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format: 
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
VpCD00340424
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1281nts
Open reading frame: 1 to 1281

ATGGACGACATATCTACGGGTATCTTATTTGCGCTACTCGCGTGTCTTATCGTCATATCA
GGTTATTTTTCTGGTTCAGAAACAGGGATGATGTCTTTGAACCGTTACCGCTTGAAGCAC
TTAGCGAAAAGTGGGCATAAGGGCGCCAAACGTGTCGAAAAGTTATTAGACCGACCAGAT
CGCTTGATTGGTCTTATTCTTATCGGCAACAACCTCGTGAACATTCTTGCTTCAGCAATT
GCGACCATCATCGGCATGCGCCTATACGGCGATTTAGGTGTCGCGATCGCAACGGGTGCG
CTAACTATGGTTGTCTTGGTGTTTGCCGAAGTCACACCAAAAACCGTTGCAGCGCTGTAT
CCAGAGCGTGTTTCCTACGCGAGCAGCATCTTGCTCACTATCCTAATGAAAGTGCTATCA
CCGCTCGTAATGCTGGTGAACTTTATTACTAATGGTTTCATTCGTTTGCTTGGCGTGAAA
GCTGATCACACGGTAGAGGATCATCTGAGTTCGGAAGAGTTACGCACGGTCGTAAATGAA
GCTGGCGGACTTATCCCTCGTCGACACCAAGACATGTTGGTGTCTATTCTCGATCTCGAA
CACGTTACTGTAAATGACATTATGGTGCCACGTAATGAGATTACGGGTATCGATATTAAC
GATGACTGGAAATCCATTGTTCGTCAGTTAACACACTCGCCACACGGTCGAATCGTTTTT
TATCGCGACCAAATCGATGAAGTGGTTGGTATGCTGCGTTTACGTGAAGCGTACCGTTTG
ATGCTAGAAAAGAACGAGTTCAACAAAGAGACGTTACTACGCGCGGCAGATGAAGTGTAT
TACATTCCTGAAGGCACGCCGCTGAACGTACAAATGTTGAAGTTCCAACGCAACAAGCAG
CGCATCGGTCTGATCGTAGACGAGTACGGTGATATCATCGGTCTAATCACTCTTGAAGAC
ATCCTAGAAGAGATCATTGGTGAGTTCACGACGTCTATTTCTCCTAGTTTGTCTGATGAA
ATCAGCCCACAAGGCGATGGTAGTTTCTTAATTGAAGGCAGCACCAATATCCGCGATATC
AACAAAGGTTTGAAATGGGATTTACCTACCGATGGCCCTAGAACCTTGAATGGTCTGATT
TTGGAACACCTAGAAGATATTCCAGAGAGTCATTTAAGTGTCCGTGTCGCGGGCCACCCA
ATGGAAATTGTTGAAATCGAAGAAAACCGAATCAAGCTAGTTCGAGTCTATCCGAAGAAG
AAAAAATCAACGTCCAAATAA

Protein sequence predicted based on nucleotide sequence

MDDISTGILFALLACLIVISGYFSGSETGMMSLNRYRLKHLAKSGHKGAKRVEKLLDRPD
RLIGLILIGNNLVNILASAIATIIGMRLYGDLGVAIATGALTMVVLVFAEVTPKTVAALY
PERVSYASSILLTILMKVLSPLVMLVNFITNGFIRLLGVKADHTVEDHLSSEELRTVVNE
AGGLIPRRHQDMLVSILDLEHVTVNDIMVPRNEITGIDINDDWKSIVRQLTHSPHGRIVF
YRDQIDEVVGMLRLREAYRLMLEKNEFNKETLLRAADEVYYIPEGTPLNVQMLKFQRNKQ
RIGLIVDEYGDIIGLITLEDILEEIIGEFTTSISPSLSDEISPQGDGSFLIEGSTNIRDI
NKGLKWDLPTDGPRTLNGLILEHLEDIPESHLSVRVAGHPMEIVEIEENRIKLVRVYPKK
KKSTSK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t246c,T82T;t261c,R87R;
t264a,L88L;t267c,Y89Y;
t564a,R188R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.8625
Recommended expression in:
Not Applicable