feoA (P. aeruginosa )
In p11 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PA4359
Gene Name:  ferrous iron transporter A
Original Clone ID:
PA4359_1_75
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
p11
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Toronto
Cheryl Arrowsmith
PSI
Northeast Structural Genomics Consortium
PaCD00341054
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 234nts
Open reading frame: 1 to 234

ATGAGCGCATTGCAACCGTCCCGTTCCTACCGCATCACCGGTTATTCCCCCGCCATCAGC
AACGGCTATCGCCAACGCCTGTTCTCCATGGGGCTTCTGCCCGGCGCGGCGCTGCGCGTG
GTGCGCATCGCCCCGCTGGGCGACCCGATCCAGGTCGAGACCCGCCAGACCAGCCTGGCG
CTGCGGCGCAAGGACCTCGCCCTGCTGACCCTCGTCCCGCTCGACGGATCCTAA

Protein sequence predicted based on nucleotide sequence

MSALQPSRSYRITGYSPAISNGYRQRLFSMGLLPGAALRVVRIAPLGDPIQVETRQTSLA
LRRKDLALLTLVPLDGS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
ins@224,6
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in PaT89
Recommended expression in:
Not Applicable