Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
10013b9BNt5bg1
Keyword:
None
Species:
Escherichia coli O157:H7
Type:
cDNA
Vector Name:
pSGX2
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
EcCD00341576
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1500nts
Open reading frame: 1 to 1500
AAGACGTTGTTCTCCCGCTTAATTACCGTTATTGCCTGCTTTTTTATCTTCTCTGCCGCA
TGGTTTTGCCTGTGGAGTATCAGCCTGCACCTGGTTGAGCGCCCTGATATGGCGGTGCTG
TTATTTCCGTTTGGTCTGCGGCTAGGGCTAATGCTGCAATGCCCGCGCGGATACTGGCCG
GTGCTGCTGGGAGCGGAGTGGCTGCTGATTTACTGGCTAACGCAGGCGGTCGGTTTAACC
CATTTCTCCTTATTGATGATCGGTAGTTTACTGACGTTACTGCCCGTGGCGCTGATCTCG
CGCTATCGCCATCAACGTGACTGGCGCACCTTGCTGTTACAGGGTGCGGCGTTAACGGCG
GCGGCGTTGTTGCAGTCGCTGCCCTGGCTTTGGCACGGCAAAGAGTCGTGGAATGCGCTG
CTGTTAACTTTAACTGGCGGCCTGACGCTGGCCCCGATATGTCTGGTGTTCTGGCATTAT
CTCGCCAATAACACCTGGCTGCCGCTCGGGCCGTCGTTAGTTTCTCAACCGATCAACTGG
CGCGGGCGGCATCTGGTCTGGTACTTGCTGCTGTTTGTTATCAGTCTCTGGCTCCAGTTG
GGCTTGCCGGACGAACTGTCGCGCTTTACGCCATTCTGCCTGGCGCTGCCGATTATCGCG
CTGGCCTGGCACTACGGCTGGCAAGGGGCGCTGATAGCGACATTGATGAACGCCATCGCG
CTGATCGCCAGTCAAACCTGGCGCGATCATCCTGTGGATTTATTGCTCTCGCTGCTGGTG
CAAAGTCTGACAGGGTTGTTACTGGGCGCTGGCATCCAGCGGTTGCGTGAACTTAACCAG
TCGCTGCAAAAGGAACTGGCGCGCAATCAGCATCTCGCTGAACGTTTGTTAGAAACTGAA
GAGAGCGTGCGCCGTGATGTGGCGCGTGAGCTGCATGATGATATCGGCCAGACCATCACT
GCCATTCGTACTCAAGCGGGCATTGTCCAGCGACTGGCGGCAGATAACGCCAGTGTGAAG
CAGAGCGGGCAGCTCATCGAACAACTGTCGCTGGGCGTGTACGACGCGGTGCGCCGTTTG
TTAGGGCGGTTACGTCCGCGCCAGTTGGATGATCTCACCCTAGAGCAGGCCATCCGCTCA
CTGATGCGGGAAATGGAGCTGGAAGGGCGCGGCATTGTCAGCCATCTCGAATGGCGAATC
GAAGAGTCAGCGTTAAGCGAAAACCAGCGCGTGACGCTGTTTCGTGTCTGCCAGGAAGGG
CTGAATAACATTGTGAAACATGCTGATGCCAGCGCGGTCACCCTGCAAGGCTGGCTGCAG
GATGAACGGTTGATGCTGGTGATTGAAGACGATGGCAGCGGTTTACCGCCGGGTTCCGGG
CAACAAGGTTTTGGCCTCACCGGAATGCGCGAGCGCGTAACGGCGCTGGGCGGCACCTTG
ACCATTTCCTGTCTGCACGGCACGCGTGTCAGCGTTTCACTACCTCAACGCTATGTCTAA
Protein sequence predicted based on nucleotide sequence
KTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFPFGLRLGLMLQCPRGYWP VLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISRYRHQRDWRTLLLQGAALTA AALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWHYLANNTWLPLGPSLVSQPINW RGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALPIIALAWHYGWQGALIATLMNAIA LIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRLRELNQSLQKELARNQHLAERLLETE ESVRRDVARELHDDIGQTITAIRTQAGIVQRLAADNASVKQSGQLIEQLSLGVYDAVRRL LGRLRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEWRIEESALSENQRVTLFRVCQEG LNNIVKHADASAVTLQGWLQDERLMLVIEDDGSGLPPGSGQQGFGLTGMRERVTALGGTL TISCLHGTRVSVSLPQRYV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-10013b
Recommended expression in:
Not Applicable
Vector Name:
pSGX2
Synonyms:
pSGX-2, pSGX2_BN
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5328
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5097
5106
bacterial origin
ColE
ColE1 bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
N-terminal 6xHis tag
5078
5095
trxn regulatory element
lac operator
lac operator
5003
5025
