int11 (S. avermitilis MA-4680 = NBRC 14893 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  int11
Gene Name:  integrase/recombinase
Original Clone ID:
11222b1BCt3p1
Keyword:
None
Species:
Streptomyces avermitilis MA-4680 = NBRC 14893
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SaCD00341611
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 489nts
Open reading frame: 1 to 489

GCACGCCGAGTGGGGATCTACACCCGCATCTCGCGGGACGACGAGGGCGATGCGCTGGGT
GTAGCCCGCCAGCACCGAGACTGCGAGCGACTTGCAGATCTCCGTTCCTGGCAGGCCGTG
AAGGTCTATGAGGACAACGACGTATCGGCCCACAAGCGCCACGTCGTACGCGAGCAGTTC
GAACTGATGCTGAGCGACCTAGGCGCCGGCCTCATCGACGGCATCGTGGCGTACGACCTA
GACAGGCTCGCGCGGCAGCCGCGAGACCTCGAGCGGCTGATCGAGATCTTCGATGAGCGC
CCACGGCTGGAGTTCGCGACGGTCACCAACGACGTCAACCTCGGAACGCCGGACGGCCGC
ACCATGTCGCGCATCATGGTCGCCTTCGCCAACAAGTCGTCGCACGATGCCTCGCGCCGC
ATCAAGCGCAAGCATCTGGAGTTGGCCCAGCAAGGCAAGGACGAAGGGCATCATCACCAT
CACCACTGA

Protein sequence predicted based on nucleotide sequence

ARRVGIYTRISRDDEGDALGVARQHRDCERLADLRSWQAVKVYEDNDVSAHKRHVVREQF
ELMLSDLGAGLIDGIVAYDLDRLARQPRDLERLIEIFDERPRLEFATVTNDVNLGTPDGR
TMSRIMVAFANKSSHDASRRIKRKHLELAQQGKDEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t151c,Y51H
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-11222b
Recommended expression in:
Not Applicable