Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
6766.1.37_GO.17074
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pVP13
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
AtCD00341748
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 339nts
Open reading frame: 1 to 339

GAAAACCTGTACTTCCAGTCCCTGCCCCAGAGGTTGAAGAAGGCTATAACGGATAATCCA
AAGAAGCTTGGGAATTTGATTGACCTCGTGAATCTTCCATCTACTCTACGGAACTTTGTT
GGTCAATCTCAGATTTCCCGTCTGGGTTGTTTCATGCGTGTCTGGTCCTATATCAAGACC
AACAATCTTCAGGATCCTAAGAACAAGAATGTGGTGATTTGTGATGAGAAGCTCAAGAGC
ATTTTGCTCGGGAAGCAGCGAGTGGAGCTAGTCGATCTTCCTTCTCTTATCAAGTTGCAT
TTTCCCAAAGAACAAAAGAAGAATTCTCAGAAAATTTAG

Protein sequence predicted based on nucleotide sequence

ENLYFQSLPQRLKKAITDNPKKLGNLIDLVNLPSTLRNFVGQSQISRLGCFMRVWSYIKT
NNLQDPKNKNVVICDEKLKSILLGKQRVELVDLPSLIKLHFPKEQKKNSQKI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a339g,*113*
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.17074
Recommended expression in:
Not Applicable