VCA0557 (V. cholerae O1 biovar El Tor str. N16961 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VCA0557
Gene Name:  diguanylate cyclase
Original Clone ID:
11201i2BCt11p1
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
VcCD00342175
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1338nts
Open reading frame: 1 to 1338

AGTGAGGCTGTAGAGCGCCAAACCTTGACTATTGCCAACTCAAAAGCATGGAAACCCTAT
TCTTATTTGGATGAACAGGGACAGCCTTCTGGCATATTGATTGATTTTTGGTTGGCTTTT
GGTGAAGCGAATCATGTCGATATTGAATTCCAACTGATGGATTGGAATGATTCCCTAGAA
GCGGTGAAGCTTGGCAAATCCGATGTTCAAGCTGGTTTGATCCGTTCTGCTTCAAGATTA
GCGTATCTCGATTTTGCAGAACCTTTACTGACAATCGATACACAACTCTACGTACACCGC
ACGTTATTGGGCGATAAATTGGATACGCTGCTATCGGGGGCCATTAACGTCTCATTAGGT
GTAGTAAAAGGGGGATTTGAACAAGAGTTCATGCAACGAGAATATCCTCAACTTAAGTTG
ATTGAGTACGCCAACAATGAATTGATGATGTCTGCAGCAAAGCGACGAGAATTAGATGGT
TTTGTGGCCGATACTCAGGTCGCCAATTTCTATATAGTGGTTTCCAATGGCGCGAAAGAT
TTTACGCCAGTGAAGTTTCTTTATTCAGAGGAATTACGTCCAGCGGTCGCCAAAGGCAAT
AGGGATTTATTAGAGCAAGTAGAGCAGGGGTTTGCACAATTAAGTAGCAATGAGAAAAGC
CGTATTTTAAGTCGATGGGTTCATATTGAAACGATTTATCCACGTTACTTAATGCCGATT
CTCGCTTCAGGTCTCTTACTCAGTATCGTTATTTATACTCTTCAGCTACGGCGTACCGTT
CGATTGCGAACACAGCAACTTGAAGAAGCCAATCAAAAACTCTCCTATTTAGCGAAAACG
GATAGCTTGACGGACATTGCTAATCGCCGTTCGTTTTTTGAACATCTTGAAGCGGAACAA
ACACGATCAGGCAGCTTAACGTTGATGGTTTTTGATATTGATGACTTCAAAACCATTAAC
GATCGCTTTGGGCATGGCGCAGGAGATAATGCCATCTGTTTCGTGGTTGGGTGTGTGCGA
CAAGCTTTAGCATCGGATACCTACTTTGCAAGGATTGGTGGTGAAGAGTTTGCTATTGTA
GCGCGTGGTAAAAATGCAGAAGAGTCGCAGCAGTTAGCTGAGCGAATTTGCCAACGAGTT
GCAGAAAAAAAGTGGGTAGTGAATGCCCAACACTCTCTGTCACTCACCATCAGCCTAGGC
TGTGCATTTTACCTACACCCAGCTCGGCCATTCAGTTTGCACGATGCCGATAGCTTAATG
TACGAAGGAAAGCGGAATGGAAAGAACCAGGTTGTCTTTCGTACCTGGTCAGAAGGGCAT
CATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

SEAVERQTLTIANSKAWKPYSYLDEQGQPSGILIDFWLAFGEANHVDIEFQLMDWNDSLE
AVKLGKSDVQAGLIRSASRLAYLDFAEPLLTIDTQLYVHRTLLGDKLDTLLSGAINVSLG
VVKGGFEQEFMQREYPQLKLIEYANNELMMSAAKRRELDGFVADTQVANFYIVVSNGAKD
FTPVKFLYSEELRPAVAKGNRDLLEQVEQGFAQLSSNEKSRILSRWVHIETIYPRYLMPI
LASGLLLSIVIYTLQLRRTVRLRTQQLEEANQKLSYLAKTDSLTDIANRRSFFEHLEAEQ
TRSGSLTLMVFDIDDFKTINDRFGHGAGDNAICFVVGCVRQALASDTYFARIGGEEFAIV
ARGKNAEESQQLAERICQRVAEKKWVVNAQHSLSLTISLGCAFYLHPARPFSLHDADSLM
YEGKRNGKNQVVFRTWSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a659g,N220S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11201i
Recommended expression in:
Not Applicable