YPTB2519 (Y. pseudotuberculosis IP 32953 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  YPTB2519
Gene Name:  LacI family transcriptional regulator
Original Clone ID:
11235a1BCt2p1
Keyword:
None
Species:
Yersinia pseudotuberculosis IP 32953
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
YpCD00342212
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1047nts
Open reading frame: 1 to 1047

AGAGAAACGCTTAAAACCATCGCTGAAACGCTTAACGTAACGCATACAACGGTCTCGAAT
GTGTTTAATCGGCCGGAAAAAGTATCGCCTGAATTGCGAGAGAAAATTTTAGCCTATGCC
GAATCTATAAATTATCACGGTCCTGCGGGTGCTGGCCGCTTTCTGCGGACCGGAAAATGT
GGAACGCTTGGGGTCATCTTTAATGACAACCTCAGTTATGTTTTCTCTGATCGCCATGAT
ATTGCGTTGATGCGCGGTATTGCCGAAGAGTGCGAAAAGAATGGTATTAACATTGCGCTA
ATGCCATTAAAAACGAATAACGCGCTAAAAAAACGAATGATGGGTGCACTGGTCGATGGT
TATATTTTAAATGCGACCTATAACAATGACGAAGTGGTCCAGGCGGCGTTGACCAGTGGC
TTGCCGGTCATTACCACCGATATCAAACTCCAGAATTTCACCTGTGTTTCGATAGATGAC
GGGGCTGCTATGCAGGCTATTTGCCACCACTTGTTGGCAGCCGGGCATAAGAAATTCGGT
ATTGTCTCTTTCCCTGTTAAAAGGCTCCCCGGCGAGCTAACATCACTGGCTGAATTCTCC
ACTGACTATGACAATGGTGTGGCTTTTGGGCGAATTAGCGCCTGTATAAAAGCGCTGTCT
GACGCTGGAATTGATTTGCACACCAGTTATGTCGCGGAACAACCGCACAGTGAAACCGGG
GGCGAAAAAGCGGCCAAAGCGCTGCTGGCAAAGGAACCGGGTATTTCGGCTATTATTTGT
TTATCTGACCGTTTTGCATTCGGCGTTTATCAATTTTGTCAGGACCACAATATTCCCGTG
CCAAGCCAAATTGCCATTACCGGGTTTGACGATATTGATATTCAGGATCCGCCAGTTGGT
TTAACGACCATCCGCCAACCTACCGTTTTAAAAGGCCGTGAAGCCGCAAGGGCCCTTATT
TCAGGCCAATTAGACAATGATATATCTTTAGATTTTGAACTTGTCGTCCGTGATTCAGCA
GAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

RETLKTIAETLNVTHTTVSNVFNRPEKVSPELREKILAYAESINYHGPAGAGRFLRTGKC
GTLGVIFNDNLSYVFSDRHDIALMRGIAEECEKNGINIALMPLKTNNALKKRMMGALVDG
YILNATYNNDEVVQAALTSGLPVITTDIKLQNFTCVSIDDGAAMQAICHHLLAAGHKKFG
IVSFPVKRLPGELTSLAEFSTDYDNGVAFGRISACIKALSDAGIDLHTSYVAEQPHSETG
GEKAAKALLAKEPGISAIICLSDRFAFGVYQFCQDHNIPVPSQIAITGFDDIDIQDPPVG
LTTIRQPTVLKGREAARALISGQLDNDISLDFELVVRDSAEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a147g,A49A;a297g,A99A;
g300a,L100L;g336a,R112R;
g558t,V186V;c627t,F209F;
c650a,c651a,T217K;t681c,H227H;
g844a,G282S;g894a,P298P;
t912c,I304I
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-11235a
Recommended expression in:
Not Applicable