CA_C0460 (C. acetobutylicum ATCC 824 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CA_C0460
Gene Name:  hypothetical protein
Original Clone ID:
APC62572_SG154_pMCSG19
Keyword:
full length
Species:
Clostridium acetobutylicum ATCC 824
Type:
cDNA
Vector Name:
pMCSG19
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
CaCD00342311
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1329nts
Open reading frame: 1 to 1329

ATGGAAGCAGACCCTGATCCAGATAACATGATTTTACAATTTGTTTTAATAGCAGTACTT
ACTTTAGTAAATGCATTCTTTTCTGCAGCAGAGATGTCAATAGTATCTCTTAATAAAACA
AGAATGAAGATTCTTTCAGAGGAAGGAAATAGAAATGCTCAGATTTTAGAAAAATTAATT
AAAGAACCAACTAAATTTTTATCAACTGTTCAGGTAGGAATTACCTTGGCAAGCTTCTTT
TCAAGCGCCTTTGCAGCTACAGGACTTTCAGACATGTTTGGAGGTTATCTAAAGAAGATA
AACATTCCATACTCAAGTCAAGTAGCACTAATAGTAGTAACAATTGTTTTATCTTATTTT
ACGCTTGTTTTTGGAGAATTGTTTCCGAAAAGAATAGCTCTTCAAAAGTCAGAGAAAATT
GCTATGCTAGCGGCGAGACCTATACTATATGTATCTAAAATTACTGTACCGTTCGTAAAA
CTGCTTTCGGCGTCTACAAATGTTTTACTACGAATTACAGGAATGAACATGAACAAATTA
GAAGAAAAAGTATCTATAGAGGAAATTAAATCCATAGTTGAAGCAGGTCAAGAATATGGT
GTAATCAATGAATCCGAAAAAGAAATGATTGATAGTATTTTTGAGTTTGATGATAGAATA
GCAGAAGAGGTAATGACGCCTAGAACAGAAGTGTATTTAATTAATATAGATACACCTTTA
AATAAATATGTTGATGAACTTTTAGAAATTAAGTATTCACGTGTACCTGTATTTGAGGGC
GATAGTGATAATATAATAGGAATTTTATATATAAAGGATTTTATAGTAGAAGCTCGTAAG
GTTGGATTTGAGAATGTAAATATAAAAAATATAATACATTCCCCTTATTTTGTACCTGAA
AGAAAGAATATAAATGAGTTATTTAAGGAACTTCAAGCTGCTAAAAAGCATATGGCTGTG
CTTATAGACGAGTATGGTGGATTTTCTGGTATAGTTACAATAGAGGACTTAATTGAGGAA
GTAATGGGAAACATCAGTGATGAATACGATGAAGATGATAACTACATAAAAAAAATTGAT
GAGTCCACATTTATAATTAAGGGTATTATACCTATAAGAGAACTAAATGAACAATTGGAC
TTAGATTTAGATGAAGAGACTGATGATTATGACACATTAGGTGGATTACTTATCAAGCTT
ATAGGACATATTCCAGAAGATAATGAACAGCAGAGTATTAAGTATAAAGGCATCACATTT
GAAATAAAAGAAGTCAAAGAAAAGAGAATAGAAAAAGTTGAGGTTCATTTACAAAAAGAT
CTTTTGGAA

Protein sequence predicted based on nucleotide sequence

MEADPDPDNMILQFVLIAVLTLVNAFFSAAEMSIVSLNKTRMKILSEEGNRNAQILEKLI
KEPTKFLSTVQVGITLASFFSSAFAATGLSDMFGGYLKKINIPYSSQVALIVVTIVLSYF
TLVFGELFPKRIALQKSEKIAMLAARPILYVSKITVPFVKLLSASTNVLLRITGMNMNKL
EEKVSIEEIKSIVEAGQEYGVINESEKEMIDSIFEFDDRIAEEVMTPRTEVYLINIDTPL
NKYVDELLEIKYSRVPVFEGDSDNIIGILYIKDFIVEARKVGFENVNIKNIIHSPYFVPE
RKNINELFKELQAAKKHMAVLIDEYGGFSGIVTIEDLIEEVMGNISDEYDEDDNYIKKID
ESTFIIKGIIPIRELNEQLDLDLDEETDDYDTLGGLLIKLIGHIPEDNEQQSIKYKGITF
EIKEVKEKRIEKVEVHLQKDLLE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Tested_Not_Found
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC62572
Recommended expression in:
Not Applicable